Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of lysates from C2C12 cells, using Phospho-MYL9-S19 Rabbit pAb (AP0872) at 1:400 dilution. C2C12 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
| Product name | Phospho-MYL9-S19 Rabbit pAb |
|---|---|
| Catalog No. | AP0872 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic phosphorylated peptide around S19 of human MYL9 (NP_006088.2). |
|---|---|
| Sequence | ATSNV |
| Gene ID | |
| Swiss Prot | |
| Synonyms | LC20; MLC2; MRLC1; MYRL2; MLC-2C; MMIHS4; Phospho-MYL9-S19 |
| Calculated MW | 20kDa |
| Observed MW | 18kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | C2C12 treated by Calyculin A |
| Cellular location | cell cortex, cytoplasm, cytosol, myofibril, Z disc. |
To download a Certificate of Compliance, please enter your Lot number below:
Lot number
* For research use only. Not for therapeutic or diagnostic purposes.
