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Phospho-Myosin Light Chain 2-T18/S19 Rabbit mAb (AP1503)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Phospho-Myosin Light Chain 2-T18/S19 Rabbit mAb (AP1503)

Western blot analysis of HeLa, using Phospho-Myosin Light Chain 2-T18/S19 Rabbit mAb (AP1503) at 1:6000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product namePhospho-Myosin Light Chain 2-T18/S19 Rabbit mAb
Catalog No.AP1503
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC62066
Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic phosphorylated peptide around T18/S19 of human Myosin Light Chain 2(NP_006088.2).
SequenceRATSNV
Gene ID
Swiss Prot
SynonymsLC20; MLC2; MRLC1; MYRL2; MLC-2C; MMIHS4
Calculated MW20kDa
Observed MW18kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:7000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa+Calyculin A
Cellular locationcell cortex, cytoplasm, cytosol, myofibril, Z disc

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ABclonal:Western blot - Phospho-Myosin Light Chain 2-T18/S19 Rabbit mAb (AP1503)}

Western blot - Phospho-Myosin Light Chain 2-T18/S19 Rabbit mAb (AP1503)

Western blot analysis of HeLa, using Phospho-Myosin Light Chain 2-T18/S19 Rabbit mAb (AP1503) at 1:6000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

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