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Phospho-MYL9-T18/S19 Rabbit pAb (AP0955)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from HeLa cells, using Phospho-MYL9-T18/S19 Rabbit pAb (AP0955) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Phospho-MYL9-T18/S19 Rabbit pAb (AP0955)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using Phospho-MYL9-T18/S19 Rabbit pAb (AP0955) at dilution of 1:100 (20x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using Phospho-MYL9-T18/S19 Rabbit pAb (AP0955) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product name	Phospho-MYL9-T18/S19 Rabbit pAb
Catalog No.	AP0955
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around T18 & S19 of human MYL9 (NP_006088.2).
Sequence	RATSNV
Gene ID
Swiss Prot
Synonyms	LC20; MLC2; MRLC1; MYRL2; MLC-2C; MMIHS4; Phospho-MYL9-T18/S19
Calculated MW	20kDa
Observed MW	18kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000 IHC-P 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	HeLa
Cellular location	cell cortex, cytoplasm, cytosol, myofibril, Z disc.
Customer validation	WB(Homo sapiens, Mus musculus, Sus scrofa) IHC(Mus musculus)