Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human
Western blot analysis of lysates from HeLa cells, using Phospho-Myosin Light Chain 2-T18 Rabbit mAb (AP1439) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
| Product name | Phospho-Myosin Light Chain 2-T18 Rabbit mAb |
|---|---|
| Catalog No. | AP1439 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC62064 |
| Immunogen | A synthetic phosphorylated peptide around T18 of human Myosin Light Chain 2（NP_006088.2）. |
|---|---|
| Sequence | ATSNV |
| Gene ID | |
| Swiss Prot | |
| Synonyms | LC20; MLC2; MRLC1; MYRL2; MLC-2C; MMIHS4; Phospho-Myosin Light Chain 2-T18 |
| Calculated MW | 20kDa |
| Observed MW | 18kDa |
| Reactivity | Human |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | HeLa treated by Calyculin A |
| Cellular location | Cell cortex, Cytoplasm, Cytosol, Myofibril, Z disc |
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