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Phospho-PLC gamma 2 (PLCG2)-Y759 Rabbit pAb (AP0785)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from Raji cells, using Phospho-PLC gamma 2 (PLCG2)-Y759 Rabbit pAb (AP0785) at 1:2000 dilution. Raji cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name	Phospho-PLC gamma 2 (PLCG2)-Y759 Rabbit pAb
Catalog No.	AP0785
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4, 5-bisphosphate to 1D-myo-inositol 1, 4, 5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around Y759 of human PLC gamma 2 (PLCG2) (NP_002652.2).
Sequence	RMYVD
Gene ID
Swiss Prot
Synonyms	FCAS3; APLAID; PLC-IV; PLC-gamma-2; Phospho-PLC gamma 2 (PLCG2)-Y759
Calculated MW	148kDa
Observed MW	150kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Raji treated by Pervanadate
Cellular location	cytoplasm, cytosol, extracellular exosome, perinuclear region of cytoplasm, plasma membrane, ruffle membrane.
Customer validation	WB(Homo sapiens) IF(Homo sapiens)