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Phospho-Raf1-S259 Rabbit pAb (AP0497)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Phospho-Raf1-S259 Rabbit pAb (AP0497)

Western blot analysis of lysates from HeLa cells, using Phospho-Raf1-S259 Rabbit pAb (AP0497) at 1:1000 dilution. HeLa cells were treated by 10% FBS after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

ABclonal:Immunoprecipitation - Phospho-Raf1-S259 Rabbit pAb (AP0497)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg Phospho-Raf1-S259 pAb (AP0497). Western blot was performed from the immunoprecipitate using Phospho-Raf1-S259 pAb (AP0497) at a dilution of 1:1000. HeLa cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.

Overview

Product namePhospho-Raf1-S259 Rabbit pAb
Catalog No.AP0497
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
ImmunogenA synthetic phosphorylated peptide around S259 of human Phospho-Raf1-S259 (NP_002871.1).
SequenceSTSTP
Gene ID
Swiss Prot
SynonymsNS5; CRAF; Raf-1; c-Raf; CMD1NN; Phospho-Raf1-S259
Calculated MW73kDa
Observed MW73kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samplesHeLa treated by 10% FBS
Cellular locationCell membrane, Cytoplasm, Mitochondrion, Nucleus.

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ABclonal:Western blot - Phospho-Raf1-S259 Rabbit pAb (AP0497)}

Western blot - Phospho-Raf1-S259 Rabbit pAb (AP0497)

Western blot analysis of lysates from HeLa cells, using Phospho-Raf1-S259 Rabbit pAb (AP0497) at 1:1000 dilution. HeLa cells were treated by 10% FBS after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
ABclonal:Immunoprecipitation - Phospho-Raf1-S259 Rabbit pAb (AP0497)}

Immunoprecipitation - Phospho-Raf1-S259 Rabbit pAb (AP0497)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg Phospho-Raf1-S259 pAb (AP0497). Western blot was performed from the immunoprecipitate using Phospho-Raf1-S259 pAb (AP0497) at a dilution of 1:1000. HeLa cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)