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Phospho-Raf1-S621 Rabbit mAb (AP1011)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - Phospho-Raf1-S621 Rabbit mAb (AP1011)

Western blot analysis of extracts of C6 cells, using Phospho-Raf1-S621 Rabbit mAb (AP1011) at 1:1000 dilution.C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Western blot - Phospho-Raf1-S621 Rabbit mAb (AP1011)

Western blot analysis of extracts of HeLa cells, using Phospho-Raf1-S621 Rabbit mAb (AP1011) at 1:1000 dilution.HeLa cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

Overview

Product namePhospho-Raf1-S621 Rabbit mAb
Catalog No.AP1011
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1556
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
ImmunogenA synthetic phosphorylated peptide around S621 of human Phospho-Raf1-S621 (P04049).
SequenceSASEP
Gene ID
Swiss Prot
SynonymsNS5; CRAF; Raf-1; c-Raf; CMD1NN
Calculated MW73kDa
Observed MW73kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesC6, HeLa
Cellular locationCell membrane, Cytoplasm, Mitochondrion, Nucleus

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ABclonal:Western blot - Phospho-Raf1-S621 Rabbit mAb (AP1011)}

Western blot - Phospho-Raf1-S621 Rabbit mAb (AP1011)

Western blot analysis of extracts of C6 cells, using Phospho-Raf1-S621 Rabbit mAb (AP1011) at 1:1000 dilution.C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Western blot - Phospho-Raf1-S621 Rabbit mAb (AP1011)}

Western blot - Phospho-Raf1-S621 Rabbit mAb (AP1011)

Western blot analysis of extracts of HeLa cells, using Phospho-Raf1-S621 Rabbit mAb (AP1011) at 1:1000 dilution.HeLa cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

* For research use only. Not for therapeutic or diagnostic purposes.

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