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Phospho-Raf1-S338 Rabbit pAb (AP0498)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Raf1-S338 pAb (AP0498) at 1:1000 dilution or Phospho-Raf1-S338 antibody (A0223). NIH/3T3 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - Phospho-Raf1-S338 Rabbit pAb (AP0498)

Immunohistochemistry analysis of paraffin-embedded Human placenta using Phospho-Raf1-S338 Rabbit pAb (AP0498) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse lung using Phospho-Raf1-S338 Rabbit pAb (AP0498) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat lung using Phospho-Raf1-S338 Rabbit pAb (AP0498) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product name	Phospho-Raf1-S338 Rabbit pAb
Catalog No.	AP0498
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S338 of human Phospho-Raf1-S338 (NP_002871.1).
Sequence	RDSSY
Gene ID
Swiss Prot
Synonyms	NS5; CRAF; Raf-1; c-Raf; CMD1NN; Phospho-Raf1-S338
Calculated MW	73kDa
Observed MW	73kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	NIH/3T3 treated by PMA
Cellular location	Cell membrane, Cytoplasm, Mitochondrion, Nucleus.
Customer validation	WB(Homo sapiens, Rattus norvegicus, Mus musculus)