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Phospho-TSC2-S1387 Rabbit pAb (AP1117)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from C2C12 cells, using Phospho-TSC2-S1387 Rabbit pAb (AP1117) at 1:1000 dilution. C2C12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. C2C12 cells were treated by AICAR (0. 5 mM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product name	Phospho-TSC2-S1387 Rabbit pAb
Catalog No.	AP1117
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene is a tumor suppressor gene that encodes the growth inhibitory protein tuberin. Tuberin interacts with hamartin to form the TSC protein complex which functions in the control of cell growth. This TSC protein complex negatively regulates mammalian target of rapamycin complex 1 (mTORC1) signaling which is a major regulator of anabolic cell growth. Mutations in this gene have been associated with tuberous sclerosis and lymphangioleiomyomatosis.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S1387 of human Tuberin/TSC2 (NP_000539.2).
Sequence	SSSSP
Gene ID
Swiss Prot
Synonyms	LAM; TSC4; PPP1R160; Phospho-TSC2-S1387
Calculated MW	201kDa
Observed MW	200kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	C2C12 treated by AICAR
Cellular location	Cytoplasm, Membrane, Peripheral membrane protein.
Customer validation	IF(Homo sapiens) WB(Homo sapiens)