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Phospho-TSC2-T1462 Rabbit pAb (AP0866)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of lysates from MCF7 cells, using Phospho-TSC2-T1462 Rabbit pAb (AP0866) at 1:1000 dilution. MCF7 cells were treated by IGF-1 (50 ng/ml) at 37℃ for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product name	Phospho-TSC2-T1462 Rabbit pAb
Catalog No.	AP0866
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene is a tumor suppressor gene that encodes the growth inhibitory protein tuberin. Tuberin interacts with hamartin to form the TSC protein complex which functions in the control of cell growth. This TSC protein complex negatively regulates mammalian target of rapamycin complex 1 (mTORC1) signaling which is a major regulator of anabolic cell growth. Mutations in this gene have been associated with tuberous sclerosis and lymphangioleiomyomatosis.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around T1462 of human TSC2 (NP_000539.2).
Sequence	GYTIS
Gene ID
Swiss Prot
Synonyms	LAM; TSC4; PPP1R160; Phospho-TSC2-T1462
Calculated MW	201kDa
Observed MW	200kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	MCF7 treated by IGF-1
Cellular location	Cytoplasm, Membrane, Peripheral membrane protein.
Customer validation	WB(Rattus norvegicus, Homo sapiens, Sus scrofa, Mus musculus) IF(Homo sapiens) WB(Homo sapiens)