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Phospho-c-Fos-T232 Rabbit pAb (AP0038)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from HepG2 cells, using Phospho-c-Fos-T232 Rabbit pAb (AP0038) at 1:1000 dilution or c-Fos antibody (A17351).HepG2 cells were treated by 10% FBS for after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Overview

Product name	Phospho-c-Fos-T232 Rabbit pAb
Catalog No.	AP0038
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around T232 of human c-Fos (NP_005243.1).
Sequence	VATPE
Gene ID
Swiss Prot
Synonyms	p55; AP-1; C-FOS; Phospho-c-Fos-T232
Calculated MW	41kDa
Observed MW	62kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HepG2 treated by FBS
Cellular location	Cytoplasm, Endoplasmic reticulum, Nucleus, cytosol
Customer validation	WB(Gallus gallus, Mus musculus, Rattus norvegicus) WB (Mus musculus)