제품 > 항체 > 다중클론항체(pAb)

STING/TMEM173 Rabbit pAb (A3575)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using STING/TMEM173 Rabbit pAb (A3575) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - STING/TMEM173 Rabbit pAb (A3575)

Immunofluorescence analysis of paraffin-embedded mouse spleen using STING/TMEM173 Rabbit pAb (A3575) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Immunofluorescence analysis of paraffin-embedded rat spleen using STING/TMEM173 Rabbit pAb (A3575) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunoprecipitation - STING/TMEM173 Rabbit pAb (A3575)

Immunoprecipitation analysis of 300 μg extracts of HT-29 cells using 3 μg STING/TMEM173 antibody (A3575). Western blot was performed from the immunoprecipitate using STING/TMEM173 antibody (A3575) at a dilution of 1:500.

Review

Overview

Product name	STING/TMEM173 Rabbit pAb
Catalog No.	A3575
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a five transmembrane protein that functions as a major regulator of the innate immune response to viral and bacterial infections. The encoded protein is a pattern recognition receptor that detects cytosolic nucleic acids and transmits signals that activate type I interferon responses. The encoded protein has also been shown to play a role in apoptotic signaling by associating with type II major histocompatibility complex. Mutations in this gene are the cause of infantile-onset STING-associated vasculopathy. Alternate splicing results in multiple transcript variants.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 139-379 of human STING/TMEM173 (NP_938023.1).
Sequence	LAPAEISAVCEKGNFNVAHGLAWSYYIGYLRLILPELQARIRTYNQHYNNLLRGAVSQRLYILLPLDCGVPDNLSMADPNIRFLDKLPQQTGDHAGIKDRVYSNSIYELLENGQRAGTCVLEYATPLQTLFAMSQYSQAGFSREDRLEQAKLFCRTLEDILADAPESQNNCRLIAYQEPADDSSFSLSQEVLRHLRQEEKEEVTVGSLKTSAVPSTSTMSQEPELLISGMEKPLPLRTDFS
Gene ID
Swiss Prot
Synonyms	ERIS; MITA; MPYS; SAVI; NET23; STING; hMITA; hSTING; TMEM173; STING-beta; STING/TMEM173
Calculated MW	42kDa
Observed MW	40kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:100 - 1:500 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	THP-1, Mouse spleen, Mouse thymus, Rat thymus, Rat lung
Cellular location	Cell membrane, Cytoplasm, Endoplasmic reticulum membrane, Mitochondrion outer membrane, Multi-pass membrane protein, perinuclear region.
Customer validation	(HT-29 cells, THP1, Mouse MEF) WB(Cynoglossus semilaevis, Mus musculus, Homo sapiens, Mus musculus, Rattus norvegicus) IP(Mus musculus) IF(Rattus norvegicus) IHC(Homo sapiens, Mus musculus) IF(Homo sapiens, Mus musculus, Rattus norvegicus) IP(Homo sapiens, Mus musculus) IHC(Homo sapiens)

Documents

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Research Area

STING/TMEM173 Rabbit pAb images

Western blot - STING/TMEM173 Rabbit pAb (A3575)

Immunofluorescence - STING/TMEM173 Rabbit pAb (A3575)

Immunoprecipitation - STING/TMEM173 Rabbit pAb (A3575)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A3575

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Reviews List

Product Protocols

References for STING/TMEM173 Rabbit pAb (A3575)

Product:

STING/TMEM173 Rabbit pAb

Journal:

Veterinary microbiology

Application:

IF:

3.293

Species:

Cynoglossus semilaevis

PMID:

30827402

Title:

In vivo cellular and molecular study on duck spleen infected by duck Tembusu virus.

References for STING/TMEM173 Rabbit pAb (A3575)

Product:

STING/TMEM173 Rabbit pAb

Journal:

Theranostics

Application:

IF:

11.556

Species:

Mus musculus

PMID:

32863951

Title:

HDAC3 inhibition ameliorates ischemia/reperfusion-induced brain injury by regulating the microglial cGAS-STING pathway

References for STING/TMEM173 Rabbit pAb (A3575)

Product:

STING/TMEM173 Rabbit pAb

Journal:

J Cell Mol Med

Application:

IF:

5.31

Species:

Rattus norvegicus

PMID:

34477314

Title:

A novel rat model of vertebral inflammation-induced intervertebral disc degeneration mediated by activating cGAS/STING molecular pathway

References for STING/TMEM173 Rabbit pAb (A3575)

Product:

STING/TMEM173 Rabbit pAb

Journal:

International journal of biological sciences

Application:

IF:

6.58

Species:

Mus musculus

PMID:

35002511

Title:

Rocaglamide promotes the infiltration and antitumor immunity of NK cells by activating cGAS-STING signaling in non-small cell lung cancer

References for STING/TMEM173 Rabbit pAb (A3575)

Product:

STING/TMEM173 Rabbit pAb

Journal:

EMBO reports

Application:

IF:

9.07

Species:

Homo sapiens

PMID:

36125406

Title:

UNC13D inhibits STING signaling by attenuating its oligomerization on the endoplasmic reticulum

References for STING/TMEM173 Rabbit pAb (A3575)

Product:

STING/TMEM173 Rabbit pAb

Journal:

Redox biology

Application:

IF:

10.78

Species:

Mus musculus

PMID:

35724543

Title:

STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice - PMC

References for STING/TMEM173 Rabbit pAb (A3575)

Product:

STING/TMEM173 Rabbit pAb

Journal:

Pharmacological research

Application:

IF:

10.33

Species:

Homo sapiens

PMID:

36464146

Title:

Oroxylin A regulates cGAS DNA hypermethylation induced by methionine metabolism to promote HSC senescence

References for STING/TMEM173 Rabbit pAb (A3575)

Product:

STING/TMEM173 Rabbit pAb

Journal:

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Application:

IF:

17.52

Species:

Mus musculus

PMID:

36721037

Title:

Loss of RPA1 Impairs Peripheral T Cell Homeostasis and Exacerbates Inflammatory Damage through Triggering T Cell Necroptosis

References for STING/TMEM173 Rabbit pAb (A3575)

Product:

STING/TMEM173 Rabbit pAb

Journal:

Cell reports

Application:

IF:

Species:

Homo sapiens

PMID:

36933219

Title:

ZYG11B potentiates the antiviral innate immune response by enhancing cGAS-DNA binding and condensation

References for STING/TMEM173 Rabbit pAb (A3575)

Product:

STING/TMEM173 Rabbit pAb

Journal:

Signal transduction and targeted therapy

Application:

IF:

38.1

Species:

Homo sapiens

PMID:

37743418

Title:

Non-small cell lung cancers (NSCLCs) oncolysis using coxsackievirus B5 and synergistic DNA-damage response inhibitors - PMC

References for STING/TMEM173 Rabbit pAb (A3575)

Product:

STING/TMEM173 Rabbit pAb

Journal:

Inflamm Bowel Dis

Application:

WB, IHC, IF, IP

IF:

7.29

Species:

Homo sapiens, Mus musculus

PMID:

34473281

Title:

STING-mediated Syk Signaling Attenuates Tumorigenesis of Colitis?associated Colorectal Cancer Through Enhancing Intestinal Epithelium Pyroptosis

References for STING/TMEM173 Rabbit pAb (A3575)

Product:

STING/TMEM173 Rabbit pAb

Journal:

PLoS pathogens

Application:

WB, IP

IF:

7.46

Species:

Homo sapiens

PMID:

36449507

Title:

MITA oligomerization upon viral infection is dependent on its N-glycosylation mediated by DDOST - PMC

References for STING/TMEM173 Rabbit pAb (A3575)

Product:

STING/TMEM173 Rabbit pAb

Journal:

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Application:

IHC

IF:

5.83

Species:

Homo sapiens

PMID:

38085169

Title:

Fusobacterium nucleatum exacerbates the progression of pulpitis by regulating the STINGdependent pathway

References for STING/TMEM173 Rabbit pAb (A3575)

Product:

STING/TMEM173 Rabbit pAb

Journal:

mBio

Application:

WB, IP

IF:

7.786

Species:

Mus musculus

PMID:

34933455

Title:

STING-Mediated Interferon Induction by Herpes Simplex Virus 1 Requires the Protein Tyrosine Kinase Syk

References for STING/TMEM173 Rabbit pAb (A3575)

Product:

STING/TMEM173 Rabbit pAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

IF:

7.41

Species:

Mus musculus

PMID:

37086511

Title:

Oroxylin A activates ferritinophagy to induce hepatic stellate cell senescence against hepatic fibrosis by regulating cGAS-STING pathway

References for STING/TMEM173 Rabbit pAb (A3575)

Product:

STING/TMEM173 Rabbit pAb

Journal:

Neuropharmacology

Application:

WB, IF

IF:

5.27

Species:

Rattus norvegicus

PMID:

35926582

Title:

Pharmacological inhibition of the cGAS-STING signaling pathway suppresses microglial M1-polarization in the spinal cord and attenuates neuropathic pain

References for STING/TMEM173 Rabbit pAb (A3575)

Product:

STING/TMEM173 Rabbit pAb

Journal:

Cellular & molecular immunology

Application:

IF:

24.1

Species:

Mus musculus

PMID:

38267694