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Phospho-STING/TMEM173-S366 Rabbit mAb (AP1369)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from THP-1 cells, using Phospho-STING/TMEM173-S366 Rabbit mAb (AP1369) at 1:1000 dilution. THP-1 cells were transfection with poly(dA:dT) (5μg/mL) for 3 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product name	Phospho-STING/TMEM173-S366 Rabbit mAb
Catalog No.	AP1369
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC57910

Background

This gene encodes a five transmembrane protein that functions as a major regulator of the innate immune response to viral and bacterial infections. The encoded protein is a pattern recognition receptor that detects cytosolic nucleic acids and transmits signals that activate type I interferon responses. The encoded protein has also been shown to play a role in apoptotic signaling by associating with type II major histocompatibility complex. Mutations in this gene are the cause of infantile-onset STING-associated vasculopathy. Alternate splicing results in multiple transcript variants.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S366 of human STING1 (NP_938023.1).
Sequence	LISGM
Gene ID
Swiss Prot
Synonyms	ERIS; MITA; MPYS; SAVI; NET23; STING; hMITA; hSTING; TMEM173; STING-beta; Phospho-STING/TMEM173-S366
Calculated MW	42kDa
Observed MW	40kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	THP-1
Cellular location	Cell membrane, Cytoplasm, Endoplasmic reticulum membrane, Mitochondrion outer membrane, Multi-pass membrane protein, perinuclear region.
Customer validation	Other(Mus musculus) WB(Mus musculus)