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Phospho-ERK1-T202 + ERK2-T185 Rabbit pAb (AP0234)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Phospho-ERK1-T202 + ERK2-T185 Rabbit pAb (AP0234) at 1:1000 dilution. Jurkat and NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight. Jurkat cells were treated by ATP(5 mM) at 30℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Phospho-ERK1-T202 + ERK2-T185 Rabbit pAb (AP0234)

Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue, using Phospho-ERK1-T202 + ERK2-T185 Rabbit pAb (AP0234). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - Phospho-ERK1-T202 + ERK2-T185 Rabbit pAb (AP0234)

Immunofluorescence analysis of methanol-fixed HeLa cells showing cytoplasmic, nuclear staining using Phospho-ERK1-T202 + ERK2-T185 Rabbit pAb (AP0234).

Overview

Product name	Phospho-ERK1-T202 + ERK2-T185 Rabbit pAb
Catalog No.	AP0234
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around T202 of human ERK1/ERK2 (NP_002737.2).
Sequence	FLTEY
Gene ID
Swiss Prot
Synonyms
Calculated MW	38kDa/40kDa/43kDa
Observed MW	42kDa/44kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:100 IF/ICC 1:20 - 1:100
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	Jurkat treated by ATP, NIH/3T3 treated by PMA
Cellular location	Cytoplasm, Nucleus.
Customer validation	WB(Gallus gallus, Mus musculus ) IHC(Mus musculus)

Documents

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Research Area

Phospho-ERK1-T202 + ERK2-T185 Rabbit pAb images

Western blot - Phospho-ERK1-T202 + ERK2-T185 Rabbit pAb (AP0234)

Immunohistochemistry - Phospho-ERK1-T202 + ERK2-T185 Rabbit pAb (AP0234)

Immunofluorescence - Phospho-ERK1-T202 + ERK2-T185 Rabbit pAb (AP0234)

Immunofluorescence analysis of methanol-fixed HeLa cells showing cytoplasmic, nuclear staining using Phospho-ERK1-T202 + ERK2-T185 Rabbit pAb (AP0234).

* For research use only. Not for therapeutic or diagnostic purposes.