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Phospho-STING/TMEM173-S366 Rabbit pAb (AP1223)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from THP-1 cells using Phospho-STING/TMEM173-S366 Rabbit pAb (AP1223) at 1:500 dilution. THP-1 cells were differentiation with TPA (200 nM) for 15 minutes and transfection with poly(dA:dT) (5ug/mL) for 3 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Overview

Product name	Phospho-STING/TMEM173-S366 Rabbit pAb
Catalog No.	AP1223
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a five transmembrane protein that functions as a major regulator of the innate immune response to viral and bacterial infections. The encoded protein is a pattern recognition receptor that detects cytosolic nucleic acids and transmits signals that activate type I interferon responses. The encoded protein has also been shown to play a role in apoptotic signaling by associating with type II major histocompatibility complex. Mutations in this gene are the cause of infantile-onset STING-associated vasculopathy. Alternate splicing results in multiple transcript variants.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S366 of human STING/TMEM173 (NP_938023.1).
Sequence	LISGME
Gene ID
Swiss Prot
Synonyms	ERIS; MITA; MPYS; SAVI; NET23; STING; hMITA; hSTING; TMEM173; STING-beta; Phospho-STING/TMEM173-S366
Calculated MW	42kDa
Observed MW	40kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	THP-1
Cellular location	autophagosome, cytosol, endosome, mitochondrial outer membrane, nucleoplasm, perinuclear region of cytoplasm, peroxisome, plasma membrane.
Customer validation	WB(Homo sapiens, Mus musculus) ELISA(Rattus norvegicus)