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ABflo® 488 Rabbit anti-Human/Mouse BCL6 mAb (A23847)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human/Mouse BCL6 mAb (A23847)

Flow cytometry: 1X10^6 HAP1 cells(Low Expression, left) and Daudi cells(right) were intracellularly-stained with ABflo® 488 Rabbit anti-Human/Mouse BCL6 mAb(A23847,5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line).Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human/Mouse BCL6 mAb (A23847)

Flow cytometry: 1X10^6 A20 cells were intracellularly-stained with ABflo® 488 Rabbit anti-Human/Mouse BCL6 mAb(A23847,5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line).Non-fluorescently stained A20 cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human/Mouse BCL6 mAb (A23847)

Flow cytometry:1X10^6 Daudi cells were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human/Mouse BCL6 mAb.

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human/Mouse BCL6 mAb (A23847)

Flow cytometry:1X10^6 A20 cells were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human/Mouse BCL6 mAb.

Overview

Product nameABflo® 488 Rabbit anti-Human/Mouse BCL6 mAb
Catalog No.A23847
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC57886-ABflo488
The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of STAT-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 235-350 of human BCL6(NP_001124317.1).
SequenceARPVPGEYSRPTLEVSPNVCHSNIYSPKETIPEEARSDMHYSVAEGLKPAAPSARNAPYFPCDKASKEEERPSSEDEIALHFEPPNAPLNRKGLVSPQSPQKSDCQPNSPTESCSS
Gene ID
Swiss Prot
SynonymsBCL6; BCL5; BCL6A; LAZ3; ZBTB27; ZNF51; B-cell CLL/lymphoma 6
Calculated MW72kDa/78kDa
Observed MW
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC (intra) 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application
Positive samples
Cellular locationNucleus

Documents

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ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human/Mouse BCL6 mAb (A23847)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human/Mouse BCL6 mAb (A23847)

Flow cytometry: 1X10^6 HAP1 cells(Low Expression, left) and Daudi cells(right) were intracellularly-stained with ABflo® 488 Rabbit anti-Human/Mouse BCL6 mAb(A23847,5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line).Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human/Mouse BCL6 mAb (A23847)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human/Mouse BCL6 mAb (A23847)

Flow cytometry: 1X10^6 A20 cells were intracellularly-stained with ABflo® 488 Rabbit anti-Human/Mouse BCL6 mAb(A23847,5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line).Non-fluorescently stained A20 cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human/Mouse BCL6 mAb (A23847)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human/Mouse BCL6 mAb (A23847)

Flow cytometry:1X10^6 Daudi cells were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human/Mouse BCL6 mAb.
ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human/Mouse BCL6 mAb (A23847)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human/Mouse BCL6 mAb (A23847)

Flow cytometry:1X10^6 A20 cells were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human/Mouse BCL6 mAb.

* For research use only. Not for therapeutic or diagnostic purposes.

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