제품 > 항체 > 단일클론항체(mAb)

ABflo® 488 Rabbit anti-Human TROP-2 mAb (A22577)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Immunofluorescence analysis of MCF7 cells using ABflo® 488 Rabbit anti-Human TROP-2 mAb (A22577) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.

Confocal imaging of A-431 cells using ABflo® 488 Rabbit anti-Human TROP-2 mAb (A22577, dilution 1:100).DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human TROP-2 mAb (A22577)

Flow cytometry:1X10^6 U-118MG cells (negative control, left) and MCF7 cells (right) were surface-stained with ABflo® 488 Rabbit anti-Human TROP-2 mAb(A22577, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Overview

Product name	ABflo® 488 Rabbit anti-Human TROP-2 mAb
Catalog No.	A22577
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC51505-ABf488

Background

This intronless gene encodes a carcinoma-associated antigen. This antigen is a cell surface receptor that transduces calcium signals. Mutations of this gene have been associated with gelatinous drop-like corneal dystrophy.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 27-274 of human TROP-2 (NP_002344.2).
Sequence	HTAAQDNCTCPTNKMTVCSPDGPGGRCQCRALGSGMAVDCSTLTSKCLLLKARMSAPKNARTLVRPSEHALVDNDGLYDPDCDPEGRFKARQCNQTSVCWCVNSVGVRRTDKGDLSLRCDELVRTHHILIDLRHRPTAGAFNHSDLDAELRRLFRERYRLHPKFVAAVHYEQPTIQIELRQNTSQKAAGDVDIGDAAYYFERDIKGESLFQGRGGLDLRVRGEPLQVERTLIYYLDEIPPKFSMKRLT
Gene ID
Swiss Prot
Synonyms	EGP1; GP50; M1S1; EGP-1; TROP2; GA7331; GA733-1
Calculated MW	36kDa
Observed MW	Refer to figures

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	IF/ICC 1:50 - 1:100 FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Immunofluorescence Flow Cytometry
Positive samples
Cellular location	Membrane, Single-pass type I membrane protein

Documents

Certificate of Compliance

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Research Area

ABflo® 488 Rabbit anti-Human TROP-2 mAb images

Immunofluorescence - ABflo® 488 Rabbit anti-Human TROP-2 mAb (A22577)

Immunofluorescence analysis of MCF7 cells using ABflo® 488 Rabbit anti-Human TROP-2 mAb (A22577) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence - ABflo® 488 Rabbit anti-Human TROP-2 mAb (A22577)

Confocal imaging of A-431 cells using ABflo® 488 Rabbit anti-Human TROP-2 mAb (A22577, dilution 1:100).DAPI was used for nuclear staining (Blue). Objective: 100x.

Flow CytoMetry - ABflo® 488 Rabbit anti-Human TROP-2 mAb (A22577)

* For research use only. Not for therapeutic or diagnostic purposes.