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ABflo® 594 Rabbit anti-Mouse CD69 mAb (A24940)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Mouse CD69 mAb (A24940)

Flow cytometry: 1X10^6 NIH/3T3 cells (negative control, left) and CTLL-2 cells (right) were surface-stained with ABflo® 594 Rabbit anti-Mouse CD69 mAb (A24940, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Mouse CD69 mAb (A24940)

Flow cytometry: 1X10^6 CTLL-2 cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Mouse CD69 mAb (A24940, 5 μl/Test, right).

Overview

Product nameABflo® 594 Rabbit anti-Mouse CD69 mAb
Catalog No.A24940
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC64627-ABflo594
Predicted to enable calcium ion binding activity and identical protein binding activity. Located in external side of plasma membrane. Orthologous to human CD69 (CD69 molecule).
ImmunogenRecombinant fusion protein containing a sequence corresponding to aminoacids 64-199 of mouse CD69(NP_001028294.1).
SequenceGKYNCPGLYEKLESSDHHVATCKNEWISYKRTCYFFSTTTKSWALAQRSCSEDAATLAVIDSEKDMTFLKRYSGELEHWIGLKNEANQTWKWANGKEFNSWFNLTGSGRCVSVNHKNVTAVDCEANFHWVCSKPSR
Gene ID
Swiss Prot
SynonymsAIM; VEA; 5830438K24Rik
Calculated MW22kDa
Observed MW
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationMembrane , Single-pass type II membrane protein.

Documents

Certificate of Compliance

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    ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Mouse CD69 mAb (A24940)}

    Flow CytoMetry - ABflo® 594 Rabbit anti-Mouse CD69 mAb (A24940)

    Flow cytometry: 1X10^6 NIH/3T3 cells (negative control, left) and CTLL-2 cells (right) were surface-stained with ABflo® 594 Rabbit anti-Mouse CD69 mAb (A24940, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Mouse CD69 mAb (A24940)}

    Flow CytoMetry - ABflo® 594 Rabbit anti-Mouse CD69 mAb (A24940)

    Flow cytometry: 1X10^6 CTLL-2 cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Mouse CD69 mAb (A24940, 5 μl/Test, right).

    * For research use only. Not for therapeutic or diagnostic purposes.

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