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PerCP/Cyanine5.5 Rabbit anti-Mouse CD69 mAb (A27283)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Flow CytoMetry - PerCP/Cyanine5.5 Rabbit anti-Mouse CD69 mAb (A27283)

Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes (treated with 10ng/mL PMA for 5 hours, right) or C57BL/6 mouse splenocytes (untreated, left) were surface-stained with PerCP/Cyanine5.5 Rabbit anti-Mouse CD69 mAb (A27283, 5 μl/Test).

Overview

Product namePerCP/Cyanine5.5 Rabbit anti-Mouse CD69 mAb
Catalog No.A27283
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC64627-PerCP-Cy5.5
Predicted to enable calcium ion binding activity and identical protein binding activity. Located in external side of plasma membrane. Orthologous to human CD69 (CD69 molecule).
ImmunogenRecombinant fusion protein containing a sequence corresponding to aminoacids 64-199 of mouse CD69 (NP_001028294.1).
SequenceGKYNCPGLYEKLESSDHHVATCKNEWISYKRTCYFFSTTTKSWALAQRSCSEDAATLAVIDSEKDMTFLKRYSGELEHWIGLKNEANQTWKWANGKEFNSWFNLTGSGRCVSVNHKNVTAVDCEANFHWVCSKPSR
Gene ID
Swiss Prot
SynonymsAIM; VEA; 5830438K24Rik
Calculated MW22kDa
Observed MW
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationMembrane, Single-pass type II membrane protein.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Flow CytoMetry - PerCP/Cyanine5.5 Rabbit anti-Mouse CD69 mAb (A27283)}

    Flow CytoMetry - PerCP/Cyanine5.5 Rabbit anti-Mouse CD69 mAb (A27283)

    Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes (treated with 10ng/mL PMA for 5 hours, right) or C57BL/6 mouse splenocytes (untreated, left) were surface-stained with PerCP/Cyanine5.5 Rabbit anti-Mouse CD69 mAb (A27283, 5 μl/Test).

    * For research use only. Not for therapeutic or diagnostic purposes.

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