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ABflo® 647 Rabbit anti-Human CD337/NKp30 mAb (A23736)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Immunofluorescence - ABflo® 647 Rabbit anti-Human CD337/NKp30 mAb (A23736)

Confocal imaging of 293T cells transfected with CD337/NKp30 using ABflo® 647 Rabbit anti-Human CD337/NKp30 mAb (A23736, dilution 1:100) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD337/NKp30 mAb (A23736)

Flow cytometry: 1X10^6 293T cells(Low Expression, left) and 293T(Transfection, right) cells were intracellularly-stained with ABflo® 647 Rabbit anti-Human CD337/NKp30 mAb(A23736, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line).Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD337/NKp30 mAb (A23736)

Flow cytometry:1X10^6 293T(Transfection) cells were intracellularly-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human CD337/NKp30 mAb(A23736, 5 μl/Test, right).

Overview

Product nameABflo® 647 Rabbit anti-Human CD337/NKp30 mAb
Catalog No.A23736
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC60018-ABf647
The protein encoded by this gene is a natural cytotoxicity receptor (NCR) that may aid NK cells in the lysis of tumor cells. The encoded protein interacts with CD3-zeta (CD247), a T-cell receptor. A single nucleotide polymorphism in the 5' untranslated region of this gene has been associated with mild malaria suceptibility. Three transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 19-135 of human CD337/NKp30 (NP_667341.1).
SequenceLWVSQPPEIRTLEGSSAFLPCSFNASQGRLAIGSVTWFRDEVVPGKEVRNGTPEFRGRLAPLASSRFLHDHQAELHIRDVRGHDASIYVCRVEVLGLGVGTGNGTRLVVEKEHPQLG
Gene ID
Swiss Prot
SynonymsNCR3; 1C7; CD337; LY117; MALS; NKp30; natural cytotoxicity triggering receptor 3
Calculated MW16kDa/17kDa/18kDa/19kDa/20kDa/21kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IF/ICC 1:50 - 1:200
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationImmunofluorescence    
Positive samples
Cellular locationPlasma membrane.

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ABclonal:Immunofluorescence - ABflo® 647 Rabbit anti-Human CD337/NKp30 mAb (A23736)}

Immunofluorescence - ABflo® 647 Rabbit anti-Human CD337/NKp30 mAb (A23736)

Confocal imaging of 293T cells transfected with CD337/NKp30 using ABflo® 647 Rabbit anti-Human CD337/NKp30 mAb (A23736, dilution 1:100) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD337/NKp30 mAb (A23736)}

Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD337/NKp30 mAb (A23736)

Flow cytometry: 1X10^6 293T cells(Low Expression, left) and 293T(Transfection, right) cells were intracellularly-stained with ABflo® 647 Rabbit anti-Human CD337/NKp30 mAb(A23736, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line).Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD337/NKp30 mAb (A23736)}

Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD337/NKp30 mAb (A23736)

Flow cytometry:1X10^6 293T(Transfection) cells were intracellularly-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human CD337/NKp30 mAb(A23736, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

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