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Biotin Rabbit anti-Human HLA-DR mAb (A27380)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 PC-3 cells (negative control, left) and Human PBMC (Lymphocytes, right) were surface-stained with Biotin Rabbit anti-Human HLA-DR mAb (A27380, 5 μl/Test, orange line) or Biotin Rabbit IgG isotype control (A25626, 5 μl/Test, blue line), followed by ABflo® 488 Streptavidin staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 Human PBMC (Lymphocytes, right) were surface-stained with Biotin Rabbit IgG isotype control (A25626, 5 μl/Test, left) or Biotin Rabbit anti-Human HLA-DR mAb (A27380, 5 μl/Test, right), followed by ABflo® 488 Streptavidin staining.

Overview

Product name	Biotin Rabbit anti-Human HLA-DR mAb
Catalog No.	A27380
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC5141-01-Biotin

Background

HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. This molecule is expressed on the surface of various antigen presenting cells such as B lymphocytes, dendritic cells, and monocytes/macrophages, and plays a central role in the immune system and response by presenting peptides derived from extracellular proteins, in particular, pathogen-derived peptides to T cells. The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.

Immunogen information

Immunogen	Recombinant protein of human HLA-DR.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	HLA-DRA1
Calculated MW	29kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Late endosome membrane, Lysosome membrane, Single-pass type I membrane protein, trans-Golgi network membrane.