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HLA-DRA Rabbit pAb (A11787)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - HLA-DRA Rabbit pAb (A11787)

Western blot analysis of various lysates using HLA-DRA Rabbit pAb (A11787) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - HLA-DRA Rabbit pAb (A11787)

Immunohistochemistry analysis of paraffin-embedded Human liver using HLA-DRA Rabbit pAb (A11787) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - HLA-DRA Rabbit pAb (A11787)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using HLA-DRA Rabbit pAb (A11787) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - HLA-DRA Rabbit pAb (A11787)

Immunohistochemistry analysis of paraffin-embedded Rat lung using HLA-DRA Rabbit pAb (A11787) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - HLA-DRA Rabbit pAb (A11787)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using HLA-DRA Rabbit pAb (A11787) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product nameHLA-DRA Rabbit pAb
Catalog No.A11787
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. This molecule is expressed on the surface of various antigen presenting cells such as B lymphocytes, dendritic cells, and monocytes/macrophages, and plays a central role in the immune system and response by presenting peptides derived from extracellular proteins, in particular, pathogen-derived peptides to T cells. The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 26-216 of human HLA-DRA (NP_061984.2).
SequenceIKEEHVIIQAEFYLNPDQSGEFMFDFDGDEIFHVDMAKKETVWRLEEFGRFASFEAQGALANIAVDKANLEIMTKRSNYTPITNVPPEVTVLTNSPVELREPNVLICFIDKFTPPVVNVTWLRNGKPVTTGVSETVFLPREDHLFRKFHYLPFLPSTEDVYDCRVEHWGLDEPLLKHWEFDAPSPLPETTE
Gene ID
Swiss Prot
SynonymsHLA-DRA1; HLA-DRA
Calculated MW29kDa
Observed MW36kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesRaji, Rat spleen
Cellular locationCell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Late endosome membrane, Lysosome membrane, Single-pass type I membrane protein, trans-Golgi network membrane.
Customer validation

IHC(Homo sapiens)

WB(Homo sapiens)

Documents

Certificate of Compliance

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    ABclonal:Western blot - HLA-DRA Rabbit pAb (A11787)}

    Western blot - HLA-DRA Rabbit pAb (A11787)

    Western blot analysis of various lysates using HLA-DRA Rabbit pAb (A11787) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - HLA-DRA Rabbit pAb (A11787)}

    Immunohistochemistry - HLA-DRA Rabbit pAb (A11787)

    Immunohistochemistry analysis of paraffin-embedded Human liver using HLA-DRA Rabbit pAb (A11787) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - HLA-DRA Rabbit pAb (A11787)}

    Immunohistochemistry - HLA-DRA Rabbit pAb (A11787)

    Immunohistochemistry analysis of paraffin-embedded Human liver cancer using HLA-DRA Rabbit pAb (A11787) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - HLA-DRA Rabbit pAb (A11787)}

    Immunohistochemistry - HLA-DRA Rabbit pAb (A11787)

    Immunohistochemistry analysis of paraffin-embedded Rat lung using HLA-DRA Rabbit pAb (A11787) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - HLA-DRA Rabbit pAb (A11787)}

    Immunohistochemistry - HLA-DRA Rabbit pAb (A11787)

    Immunohistochemistry analysis of paraffin-embedded Rat spleen using HLA-DRA Rabbit pAb (A11787) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A11787? Please let us know so that we can cite the reference in this datasheet.

    항체 (11)

    Secondary Antibodies (26)