제품 > 항체 > 단일클론항체(mAb)

HLA-DR Rabbit PolymAb® (A26694PM)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - HLA-DR Rabbit PolymAb® (A26694PM)

Western blot analysis of various lysates using HLA-DR Rabbit PolymAb® (A26694PM) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - HLA-DR Rabbit PolymAb® (A26694PM)

Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue tissue using HLA-DR Rabbit PolymAb® (A26694PM) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - HLA-DR Rabbit PolymAb® (A26694PM)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using HLA-DR Rabbit PolymAb® (A26694PM) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - HLA-DR Rabbit PolymAb® (A26694PM)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using HLA-DR Rabbit PolymAb® (A26694PM) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - HLA-DR Rabbit PolymAb® (A26694PM)

Confocal imaging of paraffin-embedded Human tonsil tissue using HLA-DR Rabbit PolymAb® (A26694PM, dilution 1:400) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Immunofluorescence - HLA-DR Rabbit PolymAb® (A26694PM)

Confocal imaging of Daudi cells using HLA-DR Rabbit PolymAb® (A26694PM, dilution 1:400) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - HLA-DR Rabbit PolymAb® (A26694PM)

Confocal imaging of Karpas-299 cells using HLA-DR Rabbit PolymAb® (A26694PM, dilution 1:400) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - HLA-DR Rabbit PolymAb® (A26694PM)

Confocal imaging of Raji cells using HLA-DR Rabbit PolymAb® (A26694PM, dilution 1:400) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product nameHLA-DR Rabbit PolymAb®
Catalog No.A26694PM
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. This molecule is expressed on the surface of various antigen presenting cells such as B lymphocytes, dendritic cells, and monocytes/macrophages, and plays a central role in the immune system and response by presenting peptides derived from extracellular proteins, in particular, pathogen-derived peptides to T cells. The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of human HLA-DRA (P01903).
SequenceKRSNYTPITNVPPEVTVLTNSPVELREPNVLICFIDKFTPPVVNVTWLRNGKPVTTGVSETVFLPREDHLFRKFHYLPFLPSTEDVYDCRVEHWGLDEPLL
Gene ID
Swiss Prot
SynonymsHLA-DRA1
Calculated MW29kDa
Observed MW30-40kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:4000
  • IHC-P 1:400 - 1:4000
  • IF/ICC 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesDaudi, U-87 MG
Cellular locationCell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Late endosome membrane, Lysosome membrane, Single-pass type I membrane protein, trans-Golgi network membrane.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - HLA-DR Rabbit PolymAb® (A26694PM)}

    Western blot - HLA-DR Rabbit PolymAb® (A26694PM)

    Western blot analysis of various lysates using HLA-DR Rabbit PolymAb® (A26694PM) at 1:1000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - HLA-DR Rabbit PolymAb® (A26694PM)}

    Immunohistochemistry - HLA-DR Rabbit PolymAb® (A26694PM)

    Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue tissue using HLA-DR Rabbit PolymAb® (A26694PM) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - HLA-DR Rabbit PolymAb® (A26694PM)}

    Immunohistochemistry - HLA-DR Rabbit PolymAb® (A26694PM)

    Immunohistochemistry analysis of paraffin-embedded Human colon tissue using HLA-DR Rabbit PolymAb® (A26694PM) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - HLA-DR Rabbit PolymAb® (A26694PM)}

    Immunohistochemistry - HLA-DR Rabbit PolymAb® (A26694PM)

    Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using HLA-DR Rabbit PolymAb® (A26694PM) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
    ABclonal:Immunofluorescence - HLA-DR Rabbit PolymAb® (A26694PM)}

    Immunofluorescence - HLA-DR Rabbit PolymAb® (A26694PM)

    Confocal imaging of paraffin-embedded Human tonsil tissue using HLA-DR Rabbit PolymAb® (A26694PM, dilution 1:400) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
    ABclonal:Immunofluorescence - HLA-DR Rabbit PolymAb® (A26694PM)}

    Immunofluorescence - HLA-DR Rabbit PolymAb® (A26694PM)

    Confocal imaging of Daudi cells using HLA-DR Rabbit PolymAb® (A26694PM, dilution 1:400) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Immunofluorescence - HLA-DR Rabbit PolymAb® (A26694PM)}

    Immunofluorescence - HLA-DR Rabbit PolymAb® (A26694PM)

    Confocal imaging of Karpas-299 cells using HLA-DR Rabbit PolymAb® (A26694PM, dilution 1:400) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Immunofluorescence - HLA-DR Rabbit PolymAb® (A26694PM)}

    Immunofluorescence - HLA-DR Rabbit PolymAb® (A26694PM)

    Confocal imaging of Raji cells using HLA-DR Rabbit PolymAb® (A26694PM, dilution 1:400) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

    * For research use only. Not for therapeutic or diagnostic purposes.

    항체 (11)

    Secondary Antibodies (26)