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PE-CF594 Rabbit anti-Human HLA-DR mAb (A27408)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - PE-CF594 Rabbit anti-Human HLA-DR mAb (A27408)

Flow cytometry: 1X10^6 PC-3 cells (negative control, left) and Human PBMC (right) were surface-stained with PE-CF594 Rabbit anti-Human HLA-DR mAb (A27408, 5 μl/Test, orange line) or PE-CF594 Rabbit IgG isotype control (5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).Cells in the Lymphocytes gate were used for analysis.

Overview

Product namePE-CF594 Rabbit anti-Human HLA-DR mAb
Catalog No.A27408
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC5141-01-PE-CF594
HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. This molecule is expressed on the surface of various antigen presenting cells such as B lymphocytes, dendritic cells, and monocytes/macrophages, and plays a central role in the immune system and response by presenting peptides derived from extracellular proteins, in particular, pathogen-derived peptides to T cells. The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.
ImmunogenRecombinant protein of human HLA-DR.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsHLA-DRA1
Calculated MW29kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Late endosome membrane, Lysosome membrane, Single-pass type I membrane protein, trans-Golgi network membrane.
    ABclonal:Flow CytoMetry - PE-CF594 Rabbit anti-Human HLA-DR mAb (A27408)}

    Flow CytoMetry - PE-CF594 Rabbit anti-Human HLA-DR mAb (A27408)

    Flow cytometry: 1X10^6 PC-3 cells (negative control, left) and Human PBMC (right) were surface-stained with PE-CF594 Rabbit anti-Human HLA-DR mAb (A27408, 5 μl/Test, orange line) or PE-CF594 Rabbit IgG isotype control (5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).Cells in the Lymphocytes gate were used for analysis.

    * For research use only. Not for therapeutic or diagnostic purposes.

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