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ESRα Rabbit mAb (A22181)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Immunofluorescence - ESRα Rabbit mAb (A22181)

Immunofluorescence analysis of C6 cells using ESRα Rabbit mAb (A22181) at dilution of 1:100(40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ESRα Rabbit mAb (A22181)

Immunofluorescence analysis of NIH/3T3 cells using ESRα Rabbit mAb (A22181) at dilution of 1:100(40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameESRα Rabbit mAb
Catalog No.A22181
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC55745
This gene encodes an estrogen receptor and ligand-activated transcription factor. The canonical protein contains an N-terminal ligand-independent transactivation domain, a central DNA binding domain, a hinge domain, and a C-terminal ligand-dependent transactivation domain. The protein localizes to the nucleus where it may form either a homodimer or a heterodimer with estrogen receptor 2. The protein encoded by this gene regulates the transcription of many estrogen-inducible genes that play a role in growth, metabolism, sexual development, gestation, and other reproductive functions and is expressed in many non-reproductive tissues. The receptor encoded by this gene plays a key role in breast cancer, endometrial cancer, and osteoporosis. This gene is reported to have dozens of transcript variants due to the use of alternate promoters and alternative splicing, however, the full-length nature of many of these variants remain uncertain.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of human ESRα (NP_000116.2).
SequenceLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVW
Gene ID
Swiss Prot
SynonymsER; ESRα
Calculated MW66kDa
Observed MWRefer to figures
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationImmunofluorescence    
Positive samples
Cellular locationCell membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic side, Golgi apparatus, Nucleus, Peripheral membrane protein, Single-pass type I membrane protein.
Customer validation

IHC(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Immunofluorescence - ESRα Rabbit mAb (A22181)}

Immunofluorescence - ESRα Rabbit mAb (A22181)

Immunofluorescence analysis of C6 cells using ESRα Rabbit mAb (A22181) at dilution of 1:100(40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ESRα Rabbit mAb (A22181)}

Immunofluorescence - ESRα Rabbit mAb (A22181)

Immunofluorescence analysis of NIH/3T3 cells using ESRα Rabbit mAb (A22181) at dilution of 1:100(40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (7)

재조합 단백질 (1)

Secondary Antibodies (26)