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Phospho-ESRα-S118 Rabbit pAb (AP0146)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Phospho-ESRα-S118 Rabbit pAb (AP0146)

Western blot analysis of lysates from MCF7 cells, using Phospho-ESRα-S118 pAb (AP0146) at 1:1000 dilution. MCF7 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product namePhospho-ESRα-S118 Rabbit pAb
Catalog No.AP0146
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an estrogen receptor and ligand-activated transcription factor. The canonical protein contains an N-terminal ligand-independent transactivation domain, a central DNA binding domain, a hinge domain, and a C-terminal ligand-dependent transactivation domain. The protein localizes to the nucleus where it may form either a homodimer or a heterodimer with estrogen receptor 2. The protein encoded by this gene regulates the transcription of many estrogen-inducible genes that play a role in growth, metabolism, sexual development, gestation, and other reproductive functions and is expressed in many non-reproductive tissues. The receptor encoded by this gene plays a key role in breast cancer, endometrial cancer, and osteoporosis. This gene is reported to have dozens of transcript variants due to the use of alternate promoters and alternative splicing, however, the full-length nature of many of these variants remain uncertain.
ImmunogenA synthetic phosphorylated peptide around S118 of human Estrogen Receptor alpha (ESR1) (NP_000116.2).
SequenceQLSPF
Gene ID
Swiss Prot
SynonymsER; ESR; Era; ESRA; ESTRR; NR3A1; Phospho-ESRα-S118
Calculated MW66kDa
Observed MW66kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:100 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMCF7 treated by Calyculin A
Cellular locationCell membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic side, Golgi apparatus, Nucleus, Peripheral membrane protein, Single-pass type I membrane protein.
ABclonal:Western blot - Phospho-ESRα-S118 Rabbit pAb (AP0146)}

Western blot - Phospho-ESRα-S118 Rabbit pAb (AP0146)

Western blot analysis of lysates from MCF7 cells, using Phospho-ESRα-S118 pAb (AP0146) at 1:1000 dilution. MCF7 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)