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ESRα Rabbit mAb (A24421)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - ESRα Rabbit mAb (A24421)

Western blot analysis of various lysates, using ESRα Rabbit mAb (A24421) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): SK-BR-3
Exposure time: 3s.

ABclonal:Chromatin Immunoprecipitation - ESRα Rabbit mAb (A24421)

Chromatin immunoprecipitation analysis of extracts of MCF7 cells treated by β-estradiol (10 nM, 45 minutes), using ESRα Rabbit mAb(A24421) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR.Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Overview

Product nameESRα Rabbit mAb
Catalog No.A24421
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC61898
This gene encodes an estrogen receptor and ligand-activated transcription factor. The canonical protein contains an N-terminal ligand-independent transactivation domain, a central DNA binding domain, a hinge domain, and a C-terminal ligand-dependent transactivation domain. The protein localizes to the nucleus where it may form either a homodimer or a heterodimer with estrogen receptor 2. The protein encoded by this gene regulates the transcription of many estrogen-inducible genes that play a role in growth, metabolism, sexual development, gestation, and other reproductive functions and is expressed in many non-reproductive tissues. The receptor encoded by this gene plays a key role in breast cancer, endometrial cancer, and osteoporosis. This gene is reported to have dozens of transcript variants due to the use of alternate promoters and alternative splicing, however, the full-length nature of many of these variants remain uncertain.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 495-595 of human ESRα (NP_000116.2).
SequenceLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV
Gene ID
Swiss Prot
SynonymsER; ESRα
Calculated MW66kDa
Observed MW66kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • IP 0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • ChIP 5μg antibody for 10μg-15μg of Chromatin
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samplesMCF7
Cellular locationCell membrane, Cytoplasm, Nucleus.

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ABclonal:Western blot - ESRα Rabbit mAb (A24421)}

Western blot - ESRα Rabbit mAb (A24421)

Western blot analysis of various lysates, using ESRα Rabbit mAb (A24421) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): SK-BR-3
Exposure time: 3s.
ABclonal:Chromatin Immunoprecipitation - ESRα Rabbit mAb (A24421)}

Chromatin Immunoprecipitation - ESRα Rabbit mAb (A24421)

Chromatin immunoprecipitation analysis of extracts of MCF7 cells treated by β-estradiol (10 nM, 45 minutes), using ESRα Rabbit mAb(A24421) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR.Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

* For research use only. Not for therapeutic or diagnostic purposes.

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