제품 > 항체 > 다중클론항체(pAb)

HSL Rabbit pAb (A15686)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of Mouse adipose, using HSL Rabbit pAb (A15686) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product name	HSL Rabbit pAb
Catalog No.	A15686
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 900-1000 of human LIPE (NP_005348.2).
Sequence	TLSPSTPSDVNFLLPPEDAGEEAEAKNELSPMDRGLGVRAAFPEGFHPRRSSQGATQMPLYSSPIVKNPFMSPLLAPDSMLKSLPPVHIVACALDPMLDDS
Gene ID
Swiss Prot
Synonyms	HSL; LHS; REH; AOMS4; FPLD6
Calculated MW	117kDa
Observed MW	84kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Mouse adipose
Cellular location	Cell membrane, Cytoplasm, Membrane, caveola, cytosol
Customer validation	WB(Mus musculus, Anas platyrhynchos, Homo sapiens) IHC(Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

HSL Rabbit pAb images

Western blot - HSL Rabbit pAb (A15686)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for HSL Rabbit pAb (A15686)

Product:

HSL Rabbit pAb

Journal:

Journal of agricultural and food chemistry

Application:

IF:

5.279

Species:

Mus musculus

PMID:

31075194

Title:

A Combination of Capsaicin and Capsiate Induces Browning in 3T3-L1 White Adipocytes via Activation of the PPARγ/ β3-AR Signaling Pathways.

References for HSL Rabbit pAb (A15686)

Product:

HSL Rabbit pAb

Journal:

Journal of molecular cell biology

Application:

IF:

8.18

Species:

Mus musculus

PMID:

36107452

Title:

Knockdown of hepatocyte Perilipin-3 mitigates hepatic steatosis and steatohepatitis caused by hepatocyte CGI-58 deletion in mice

References for HSL Rabbit pAb (A15686)

Product:

HSL Rabbit pAb

Journal:

Cell discovery

Application:

IF:

38.07

Species:

Mus musculus

PMID:

36376273

Title:

Single-cell sequencing unveils key contributions of immune cell populations in cancer-associated adipose wasting - PMC

References for HSL Rabbit pAb (A15686)

Product:

HSL Rabbit pAb

Journal:

Phytomedicine : international journal of phytotherapy and phytopharmacology

Application:

IF:

6.65

Species:

Mus musculus

PMID:

36610148

Title:

Curcumin Treatment Suppresses Cachexia-Associated Adipose Wasting in Mice by Blocking the cAMP/PKA/CREB Signaling Pathway

References for HSL Rabbit pAb (A15686)

Product:

HSL Rabbit pAb

Journal:

The American journal of Chinese medicine

Application:

IF:

Species:

Mus musculus

PMID:

35642460

Title:

Smilax china Polyphenols Stimulate Browning via [Formula: see text]3-Adrenergic Receptor/AMP-Activated Protein Kinase [Formula: see text] Signaling Pathway in 3T3-L1 Adipocytes

References for HSL Rabbit pAb (A15686)

Product:

HSL Rabbit pAb

Journal:

Nature communications

Application:

IF:

17.69

Species:

Mus musculus

PMID:

36934083

Title:

Acute liver steatosis translationally controls the epigenetic regulator MIER1 to promote liver regeneration in a study with male mice - PMC

References for HSL Rabbit pAb (A15686)

Product:

HSL Rabbit pAb

Journal:

Journal of animal science and biotechnology

Application:

IF:

Species:

Anas platyrhynchos

PMID:

35581591

Title:

Dietary methionine deficiency stunts growth and increases fat deposition via suppression of fatty acids transportation and hepatic catabolism in Pekin ducks

References for HSL Rabbit pAb (A15686)

Product:

HSL Rabbit pAb

Journal:

iScience

Application:

IF:

6.1

Species:

Mus musculus

PMID:

38303710