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Phospho-HSL-S660 Rabbit pAb (AP0853)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of lysates from 3T3-L1 cells, using Phospho-HSL-S660 Rabbit pAb (AP0853) at 1:600 dilution. 3T3-L1 cells were treated with Isoproterenol (10 µM for 20 min) at 37℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product name	Phospho-HSL-S660 Rabbit pAb
Catalog No.	AP0853
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S660 of human LIPE (NP_005348.2).
Sequence	QTSRS
Gene ID
Swiss Prot
Synonyms	HSL; LHS; REH; AOMS4; FPLD6; Phospho-HSL-S660
Calculated MW	117kDa
Observed MW	81kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	3T3-L1 treated with Isoproterenol
Cellular location	Cell membrane, Cytoplasm, Membrane, caveola, cytosol.
Customer validation	WB(Mus musculus)