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Phospho-HSL-S660 Rabbit pAb (AP1242)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from HeLa cells, using Phospho-HSL-S660 Rabbit pAb (AP1242) at 1:1000 dilution. HeLa cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Overview

Product name	Phospho-HSL-S660 Rabbit pAb
Catalog No.	AP1242
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.

Immunogen information

Immunogen	A synthesized peptide derived from human HSL (Phospho-Ser660)
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	HSL; LHS; REH; AOMS4; FPLD6; Phospho-HSL-S660
Calculated MW	117kDa
Observed MW	81kDa, 83kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.4.
Key application	Western blotting
Positive samples	HeLa treated by TNF-α
Cellular location	Cell membrane, Cytoplasm, Membrane, caveola, cytosol.
Customer validation	WB(Mus musculus)