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Phospho-HSL-S853 Rabbit mAb (AP1151)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-HSL-S853 Rabbit mAb (AP1151) at 1:1000 dilution. NIH/3T3 cells were treated by Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

Overview

Product name	Phospho-HSL-S853 Rabbit mAb
Catalog No.	AP1151
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC1657

Background

The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S853 of human HSL (Q05469).
Sequence	RRSVS
Gene ID
Swiss Prot
Synonyms	HSL; LHS; REH; AOMS4; FPLD6; Phospho-HSL-S853
Calculated MW	117kDa
Observed MW	83kDa

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	NIH/3T3 treated by Forskolin
Cellular location	Cell membrane, Cytoplasm, Membrane, caveola, cytosol.
Customer validation	WB(Mus musculus) IHC(Mus musculus)