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Phospho-HSL-S563 Rabbit pAb (AP0851)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from Mouse brown fat using Phospho-HSL-S563 Rabbit pAb (AP0851) at 1:5000 dilution incubated overnight at 4℃. Mouse brown fat were treated by λ-PP mixed solution (1 μl) at 30℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name	Phospho-HSL-S563 Rabbit pAb
Catalog No.	AP0851
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S563 of rat HSL (XP_006228456.1).
Sequence	RRSVS
Gene ID
Swiss Prot
Synonyms	HSL; LHS; REH; AOMS4; FPLD6; Phospho-HSL-S563
Calculated MW	117kDa
Observed MW	83kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Mouse brown fat
Cellular location	Cell membrane, Cytoplasm, Membrane, caveola, cytosol.
Customer validation	WB(Mus musculus, Anas platyrhynchos, Homo sapiens)