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MAP2 Rabbit pAb (A17409)

Publications (7) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Immunohistochemistry - MAP2 Rabbit pAb (A17409)

Immunohistochemistry analysis of paraffin-embedded Rat brain using MAP2 Rabbit pAb (A17409) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - MAP2 Rabbit pAb (A17409)

Immunohistochemistry analysis of paraffin-embedded Rat brain using MAP2 Rabbit pAb (A17409) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - MAP2 Rabbit pAb (A17409)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using MAP2 Rabbit pAb (A17409) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - MAP2 Rabbit pAb (A17409)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using MAP2 Rabbit pAb (A17409) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product nameMAP2 Rabbit pAb
Catalog No.A17409
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1510-1650 of human MAP2 (NP_002365.3).
SequenceGGESALAPSVFKQAKDKVSDGVTKSPEKRSSLPRPSSILPPRRGVSGDRDENSFSLNSSISSSARRTTRSEPIRRAGKSGTSTPTTPGSTAITPGTPPSYSSRTPGTPGTPSYPRTPHTPGTPKSAILVPSEKKVAIIRTP
Gene ID
Swiss Prot
SynonymsMAP-2; MAP2A; MAP2B; MAP2C; MAP2
Calculated MW200kDa
Observed MW75kDa/280kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesSH-SY5Y, PC-12
Cellular locationCytoplasm, cytoskeleton.
Customer validation

IF(Rattus norvegicus, Mus musculus)

WB(Mus musculus)

IF(Mus musculus)

Documents

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ABclonal:Immunohistochemistry - MAP2 Rabbit pAb (A17409)}

Immunohistochemistry - MAP2 Rabbit pAb (A17409)

Immunohistochemistry analysis of paraffin-embedded Rat brain using MAP2 Rabbit pAb (A17409) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - MAP2 Rabbit pAb (A17409)}

Immunohistochemistry - MAP2 Rabbit pAb (A17409)

Immunohistochemistry analysis of paraffin-embedded Rat brain using MAP2 Rabbit pAb (A17409) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - MAP2 Rabbit pAb (A17409)}

Immunohistochemistry - MAP2 Rabbit pAb (A17409)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using MAP2 Rabbit pAb (A17409) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - MAP2 Rabbit pAb (A17409)}

Immunohistochemistry - MAP2 Rabbit pAb (A17409)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using MAP2 Rabbit pAb (A17409) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A17409? Please let us know so that we can cite the reference in this datasheet.

항체 (4)

Secondary Antibodies (25)