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Phospho-p53-S33 Rabbit pAb (AP0762)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from HT-29 cells, using Phospho-p53-S33 Rabbit pAb (A3185). HT-29 cells were treated by Nocodazole (100ng/mL) for 16 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Phospho-p53-S33 Rabbit pAb (AP0762)

Immunohistochemistry analysis of paraffin-embedded Rat liver using Phospho-p53-S33 Rabbit pAb (AP0762) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human colon using Phospho-p53-S33 Rabbit pAb (AP0762) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse liver using Phospho-p53-S33 Rabbit pAb (AP0762) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunoprecipitation - Phospho-p53-S33 Rabbit pAb (AP0762)

Immunoprecipitation analysis of 200 μg extracts of HT-29 cells, using 3 μg Phospho-p53-S33 pAb (AP0762). Western blot was performed from the immunoprecipitate using Phospho-p53-S33 pAb (AP0762) at a dilution of 1:1000. HT-29 cells were treated by nocodazole (100 ng/mL) at 37℃ for 16 hours.

Review

Overview

Product name	Phospho-p53-S33 Rabbit pAb
Catalog No.	AP0762
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S33 of human p53 (NP_000537.3).
Sequence	VLSPL
Gene ID
Swiss Prot
Synonyms	P53; BCC7; LFS1; BMFS5; TRP53; Phospho-p53-S33
Calculated MW	44kDa
Observed MW	55kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:100 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunoprecipitation
Positive samples	HT-29 treated by Nocodazole
Cellular location	Cytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body
Customer validation	(Human lung cancer cell)