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Phospho-p53-S46 Rabbit pAb (AP0476)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from HeLa cells, using Phospho-p53-S46 Rabbit pAb (AP0476) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunoprecipitation - Phospho-p53-S46 Rabbit pAb (AP0476)

Immunoprecipitation analysis of 200 μg extracts of 293T cells, using 3 μg Phospho-p53-S46 pAb (AP0476). Western blot was performed from the immunoprecipitate using Phospho-p53-S46 pAb (AP0476) at a dilution of 1:1000. 293T cells were treated by UV at room temperature for 30 minutes after serum-starvation overnight, and then treated by 10% FBS at 37℃ for 30 minutes.

Overview

Product name	Phospho-p53-S46 Rabbit pAb
Catalog No.	AP0476
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S46 of human p53 (NP_000537.3).
Sequence	MLSPD
Gene ID
Swiss Prot
Synonyms	P53; BCC7; LFS1; BMFS5; TRP53; Phospho-p53-S46
Calculated MW	44kDa
Observed MW	53kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunoprecipitation
Positive samples	HeLa treated by Calyculin A
Cellular location	Cytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body.
Customer validation	WB(Homo sapiens)