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Phospho-p53-S392 Rabbit pAb (AP0860)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p53-S392 Rabbit pAb (AP0860) at 1:1000 dilution. NIH/3T3 cells were treated by Paclitaxel (100 nM/ml) at 37℃ for 20 hours. NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 1s.

Western blot analysis of various lysates using Phospho-p53-S392 Rabbit pAb (AP0860) at 1:1000 dilution. HeLa cells were treated by Paclitaxel (100 nM/ml) at 37℃ for 20 hours. HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours. C6 cells were treated by Paclitaxel (100 nM) at 37℃ for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 20s.

Overview

Product name	Phospho-p53-S392 Rabbit pAb
Catalog No.	AP0860
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S392 of human p53 (NP_000537.3).
Sequence	GPDSD
Gene ID
Swiss Prot
Synonyms	P53; BCC7; LFS1; BMFS5; TRP53; Phospho-p53-S392
Calculated MW	44kDa
Observed MW	53kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa treated by Paclitaxel, HeLa treated by Hydroxyurea, NIH/3T3 treated by Paclitaxel, NIH/3T3 treated by Hydroxyurea, C6 treated by Paclitaxel, C6 treated by Hydroxyurea
Cellular location	Cytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body.
Customer validation	WB(Homo sapiens)