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Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit mAb (AP1147)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit mAb (AP1147)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit mAb (AP1147) at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Western blot - Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit mAb (AP1147)

Western blot analysis of lysates from A-431 cells, using Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit mAb (AP1147) at 1:1000 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit mAb (AP1147)

Western blot analysis of lysates from C6 cells, using Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit mAb (AP1147) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product namePhospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit mAb
Catalog No.AP1147
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1608
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
ImmunogenA synthetic phosphorylated peptide around S380 of human p90Rsk/RSK1/RPS6KA1KS6A1 (Q15418).
SequenceGFSFV
Gene ID
Swiss Prot
SynonymsRSK; HU-1; RSK1; p90Rsk; MAPKAPK1; MAPKAPK1A; Phospho-p90Rsk/RSK1/RPS6KA1-S380
Calculated MW83kDa
Observed MW90kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesA-431 treated by EGF, NIH/3T3 treated by PDGF, C6 treated by PMA
Cellular locationCytoplasm, Nucleus.
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit mAb (AP1147)}

Western blot - Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit mAb (AP1147)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit mAb (AP1147) at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit mAb (AP1147)}

Western blot - Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit mAb (AP1147)

Western blot analysis of lysates from A-431 cells, using Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit mAb (AP1147) at 1:1000 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit mAb (AP1147)}

Western blot - Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit mAb (AP1147)

Western blot analysis of lysates from C6 cells, using Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit mAb (AP1147) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)