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Phospho-p90Rsk/RSK1/RPS6KA1-T359/S363 Rabbit pAb (AP0539)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of extracts of A431 cells, using Phospho-p90Rsk/RSK1/RPS6KA1-T359/S363 antibody (AP0539).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Overview

Product name	Phospho-p90Rsk/RSK1/RPS6KA1-T359/S363 Rabbit pAb
Catalog No.	AP0539
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around T359 & S363 of human p90Rsk/RSK1/RPS6KA1 (NP_002944.2).
Sequence	TPKDS
Gene ID
Swiss Prot
Synonyms	RSK; HU-1; RSK1; p90Rsk; MAPKAPK1; MAPKAPK1A
Calculated MW	83kDa
Observed MW	83kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	A-431
Cellular location	Cytoplasm, Nucleus
Customer validation	WB(Sus scrofa, Mus musculus, Homo sapiens, Danio rerio) IP(Mus musculus)