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α-Synuclein Rabbit PolymAb® (A22598PM)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - α-Synuclein Rabbit PolymAb® (A22598PM)

Western blot analysis of various lysates, using α-Synuclein Rabbit PolymAb® (A22598PM) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - α-Synuclein Rabbit PolymAb® (A22598PM)

Western blot analysis of various lysates, using α-Synuclein Rabbit PolymAb® (A22598PM) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - α-Synuclein Rabbit PolymAb® (A22598PM)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using α-Synuclein Rabbit PolymAb® (A22598PM) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - α-Synuclein Rabbit PolymAb® (A22598PM)

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using α-Synuclein Rabbit PolymAb® (A22598PM) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - α-Synuclein Rabbit PolymAb® (A22598PM)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using α-Synuclein Rabbit PolymAb® (A22598PM) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - α-Synuclein Rabbit PolymAb® (A22598PM)

Immunofluorescence analysis of Neuro-2a cells using α-Synuclein Rabbit PolymAb? (A22598PM) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - α-Synuclein Rabbit PolymAb® (A22598PM)

Confocal imaging of SH-SY5Y cells using α-Synuclein Rabbit PolymAb® (A22598PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - α-Synuclein Rabbit PolymAb® (A22598PM)

Confocal imaging of Neuro-2a cells using α-Synuclein Rabbit PolymAb® (A22598PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product nameα-Synuclein Rabbit PolymAb®
Catalog No.A22598PM
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 59-140 of human α-Synuclein Rabbit PolymAb® (NP_000336.1).
SequenceTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEA
Gene ID
Swiss Prot
SynonymsPD1; NACP; PARK1; PARK4
Calculated MW14kDa
Observed MW18kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:1000-1:5000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, SH-SY5Y, Mouse brain, Rat brain
Cellular locationCell junction, Cytoplasm, Membrane, Nucleus, Secreted, cytosol, Synapse.
ABclonal:Western blot - α-Synuclein Rabbit PolymAb® (A22598PM)}

Western blot - α-Synuclein Rabbit PolymAb® (A22598PM)

Western blot analysis of various lysates, using α-Synuclein Rabbit PolymAb® (A22598PM) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - α-Synuclein Rabbit PolymAb® (A22598PM)}

Western blot - α-Synuclein Rabbit PolymAb® (A22598PM)

Western blot analysis of various lysates, using α-Synuclein Rabbit PolymAb® (A22598PM) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - α-Synuclein Rabbit PolymAb® (A22598PM)}

Immunohistochemistry - α-Synuclein Rabbit PolymAb® (A22598PM)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using α-Synuclein Rabbit PolymAb® (A22598PM) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - α-Synuclein Rabbit PolymAb® (A22598PM)}

Immunohistochemistry - α-Synuclein Rabbit PolymAb® (A22598PM)

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using α-Synuclein Rabbit PolymAb® (A22598PM) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - α-Synuclein Rabbit PolymAb® (A22598PM)}

Immunohistochemistry - α-Synuclein Rabbit PolymAb® (A22598PM)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using α-Synuclein Rabbit PolymAb® (A22598PM) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - α-Synuclein Rabbit PolymAb® (A22598PM)}

Immunofluorescence - α-Synuclein Rabbit PolymAb® (A22598PM)

Immunofluorescence analysis of Neuro-2a cells using α-Synuclein Rabbit PolymAb? (A22598PM) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - α-Synuclein Rabbit PolymAb® (A22598PM)}

Immunofluorescence - α-Synuclein Rabbit PolymAb® (A22598PM)

Confocal imaging of SH-SY5Y cells using α-Synuclein Rabbit PolymAb® (A22598PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - α-Synuclein Rabbit PolymAb® (A22598PM)}

Immunofluorescence - α-Synuclein Rabbit PolymAb® (A22598PM)

Confocal imaging of Neuro-2a cells using α-Synuclein Rabbit PolymAb® (A22598PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

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