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Phospho-α-Synuclein-Y136 Rabbit pAb (AP0254)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-α-Synuclein-Y136 Rabbit pAb (AP0254)

Western blot analysis of lysates from Mouse brain, using Phospho-α-Synuclein-Y136 Rabbit pAb (AP0254) at 1:1000 dilution. Mouse brain were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product namePhospho-α-Synuclein-Y136 Rabbit pAb
Catalog No.AP0254
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene.
ImmunogenA synthetic phosphorylated peptide around Y136 of human alpha-Synuclein (NP_000336.1).
SequenceQDYEP
Gene ID
Swiss Prot
SynonymsPD1; NACP; PARK1; PARK4; Phospho-α-Synuclein-Y136
Calculated MW14kDa
Observed MW18kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse brain
Cellular locationCell junction, Cytoplasm, Membrane, Nucleus, Secreted, cytosol, synapse.

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ABclonal:Western blot - Phospho-α-Synuclein-Y136 Rabbit pAb (AP0254)}

Western blot - Phospho-α-Synuclein-Y136 Rabbit pAb (AP0254)

Western blot analysis of lysates from Mouse brain, using Phospho-α-Synuclein-Y136 Rabbit pAb (AP0254) at 1:1000 dilution. Mouse brain were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

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재조합 단백질 (1)

Secondary Antibodies (26)