제품 > 항체 > 다중클론항체(pAb)

α-Synuclein Rabbit pAb (A7215)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using α-Synuclein Rabbit pAb (A7215) at 1:1000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 1s.

Western blot analysis of lysates from 293T cells using α-Synuclein Rabbit pAb(A7215) at 1:1000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime:30s.

ABclonal:Immunofluorescence - α-Synuclein Rabbit pAb (A7215)

Immunofluorescence analysis of mouse brain cells using α-Synuclein Rabbit pAb (A7215) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of rat brain cells using α-Synuclein Rabbit pAb (A7215) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of SH-SY5Y cells using α-Synuclein Rabbit pAb (A7215) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	α-Synuclein Rabbit pAb
Catalog No.	A7215
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 59-140 of human alpha-Synuclein (NP_000336.1).
Sequence	TKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEA
Gene ID
Swiss Prot
Synonyms	PD1; NACP; PARK1; PARK4; α-Synuclein
Calculated MW	14kDa
Observed MW	18kDa/

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	293T, Mouse brain, Rat brain, Mouse brain, Rat brain, 293T
Cellular location	Cell junction, Cytoplasm, Membrane, Nucleus, Secreted, cytosol, synapse
Customer validation	WB(Homo sapiens, Mus musculus, Rattus norvegicus) IF(Mus musculus) IHC(Mus musculus)

Documents

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Research Area

α-Synuclein Rabbit pAb images

Western blot - α-Synuclein Rabbit pAb (A7215)

Immunofluorescence - α-Synuclein Rabbit pAb (A7215)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for α-Synuclein Rabbit pAb (A7215)

Product:

α-Synuclein Rabbit pAb

Journal:

Journal of proteome research

Application:

IF:

3.8

Species:

Homo sapiens

PMID:

28892615

Title:

Quantitative Proteomics Reveals the Regulatory Networks of Circular RNA CDR1as in Hepatocellular Carcinoma Cells

References for α-Synuclein Rabbit pAb (A7215)

Product:

α-Synuclein Rabbit pAb

Journal:

Frontiers in aging neuroscience

Application:

IF:

4.1

Species:

Mus musculus

PMID:

36761179

Title:

Loss of RAB39B does not alter MPTP-induced Parkinsons disease-like phenotypes in mice - PMC

References for α-Synuclein Rabbit pAb (A7215)

Product:

α-Synuclein Rabbit pAb

Journal:

Food and chemical toxicology

Application:

IF:

3.9

Species:

Homo sapiens

PMID:

30578841

Title:

Corynoxine B ameliorates HMGB1-dependent autophagy dysfunction during manganese exposure in SH-SY5Y human neuroblastoma cells.

References for α-Synuclein Rabbit pAb (A7215)

Product:

α-Synuclein Rabbit pAb

Journal:

Toxicology Letters

Application:

WB, IHC

IF:

2.9

Species:

Mus musculus

PMID:

31783120

Title:

The effect of -synuclein and Tau in methamphetamine induced neurotoxicity in vivo and in vitro

References for α-Synuclein Rabbit pAb (A7215)

Product:

α-Synuclein Rabbit pAb

Journal:

European journal of pharmacology

Application:

IF:

4.2

Species:

Homo sapiens

PMID:

38685306

Title:

MiR-29a efficiently suppresses the generation of reactive oxygen species and -synuclein in a cellular model of Parkinson's disease by potentially targeting

References for α-Synuclein Rabbit pAb (A7215)

Product:

α-Synuclein Rabbit pAb

Journal:

The International journal of neuroscience

Application:

IF:

1.7

Species:

Homo sapiens

PMID:

33045885