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Phospho-α-Synuclein-S129 Rabbit pAb (AP0450)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-α-Synuclein-S129 Rabbit pAb (AP0450)

Western blot analysis of lysates from mouse brain tissue, using Phospho-α-Synuclein-S129 Rabbit pAb (AP0450).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Overview

Product namePhospho-α-Synuclein-S129 Rabbit pAb
Catalog No.AP0450
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene.
ImmunogenA synthetic phosphorylated peptide around S129 of human alpha-Synuclein (NP_000336.1).
SequenceMPSEE
Gene ID
Swiss Prot
SynonymsPD1; NACP; PARK1; PARK4; Phospho-α-Synuclein-S129
Calculated MW14kDa
Observed MW20kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse brain
Cellular locationCell junction, Cytoplasm, Membrane, Nucleus, Secreted, cytosol, synapse

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ABclonal:Western blot - Phospho-α-Synuclein-S129 Rabbit pAb (AP0450)}

Western blot - Phospho-α-Synuclein-S129 Rabbit pAb (AP0450)

Western blot analysis of lysates from mouse brain tissue, using Phospho-α-Synuclein-S129 Rabbit pAb (AP0450).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

* For research use only. Not for therapeutic or diagnostic purposes.

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재조합 단백질 (1)

Secondary Antibodies (26)