제품 > 항체 > 단일클론항체(mAb)

[KO Validated] N-terminal alpha-synuclein mAb [MJFF-B-3A4] (A27117)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from wild type (WT) and Alpha synuclein knockout (KO) aSyn cells using [KO Validated] N-terminal alpha-synuclein mAb [MJFF-B-3A4] (A27117) at 1:5000 dilution .
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Western blot analysis of various lysates using [KO Validated] N-terminal alpha-synuclein mAb [MJFF-B-3A4] (A27117) at 1:5000 dilution .
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): T-47D
Exposure time: 60s.

ABclonal:Immunofluorescence - [KO Validated] N-terminal alpha-synuclein mAb [MJFF-B-3A4] (A27117)

Confocal imaging of Mouse primary hippocampal neuron cells(Wild type) and Mouse primary hippocampal neuron cells(SNCA KO) using N-terminal alpha-synuclein mAb [MJFF-B-3A4] (A27117, dilution 1:500) followed by a further incubation with AF488 conjugated donkey Anti-Rabbit (H+L) (A-21206, dilution 1:500) (Green). The cells were counterstained with total aSyn antibody and MAP2 antibody followed by incubation with AF647 conjugated Donkey Anti-mouse IgG (H+L) Ab (A-31571) (Red) and AF568 conjugated Donkey Anti-chicken IgG (H+L) Ab (703-545-155, dilution 1:400) (Grey).DAPI was used for nuclear staining (Blue). Objective: 40x.

Overview

Product name	[KO Validated] N-terminal alpha-synuclein mAb [MJFF-B-3A4]
Catalog No.	A27117
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC5166

Background

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Alpha-synuclein is a neuronal protein that plays many roles in synaptic activity such as membrane trafficking of synaptic vesicles and neurotransmitter release. Multiplications in SNCA have been implicated in genetic forms of Parkinson disease and pathological post-translational modifications of alpha-synuclein are a hallmark of Parkinson’s disease. These modifications include phosphorylation, nitration, acetylation, truncation, and aggregation.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence corresponding to amino acids 40-50 of human alpha-synuclein(NP_000336.1).
Sequence	VGSKTKEGVVH
Gene ID
Swiss Prot
Synonyms	NACP; PARK1; α-Synuclein; SNCA
Calculated MW	14kDa
Observed MW	18kDa/

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:5000 - 1:30000 IF/ICC 1:200 - 1:500 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	SK-MEL-28, HEL, HeLa, mouse brain, mouse liver, rat brain, HIPP Neurons, Recombinant Mouse Protein, Recombinant Human Protein
Cellular location	Cell junction, Cytoplasm, Membrane, Nucleus, Secreted, cytosol, synapse, Cell projection, axon.