제품 > 항체 > 다중클론항체(pAb)

Bcl-2 Rabbit pAb (A2845)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from Jurkat cells, using Bcl-2 Rabbit pAb (A2845) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Western blot analysis of lysates from Mouse lung, using Bcl-2 Rabbit pAb (A2845) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A2845)

Immunofluorescence analysis of L929 cells using Bcl-2 Rabbit pAb (A2845) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review

Overview

Product name	Bcl-2 Rabbit pAb
Catalog No.	A2845
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-239 of human Bcl-2 (NP_000624.2).
Sequence	MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAPAPGIFSSQPGHTPHPAASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDNIALWMTEYLNRHLHTWIQDNGGWDAFVELYGPSMRPLFDFSWLSLKTLLSLALVGACITLGAYLGHK
Gene ID
Swiss Prot
Synonyms	Bcl-2; PPP1R50
Calculated MW	26kDa
Observed MW	26kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:100 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Immunoprecipitation
Positive samples	Jurkat, Mouse lung
Cellular location	Endoplasmic reticulum membrane, Mitochondrion outer membrane, Nucleus membrane, Single-pass membrane protein.
Customer validation	(Human tumor cell) WB(Rattus norvegicus, Mus musculus, Homo sapiens, Chlorocebus aethiops)

Documents

Certificate of Compliance

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Research Area

Bcl-2 Rabbit pAb images

Western blot - Bcl-2 Rabbit pAb (A2845)

Immunofluorescence - Bcl-2 Rabbit pAb (A2845)

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for Bcl-2 Rabbit pAb (A2845)

Product:

Bcl-2 Rabbit pAb

Journal:

Basic Research in Cardiology

Application:

IF:

7.5

Species:

Rattus norvegicus

PMID:

28534118

Title:

Exercise-induced circulating extracellular vesicles protect against cardiac ischemia-reperfusion injury

References for Bcl-2 Rabbit pAb (A2845)

Product:

Bcl-2 Rabbit pAb

Journal:

Journal of Cardiovascular Translational Research

Application:

IF:

2.4

Species:

Rattus norvegicus

PMID:

29404859

Title:

Serum Exosomes Attenuate H2O2-Induced Apoptosis in Rat H9C2 Cardiomyocytes via ERK1/2

References for Bcl-2 Rabbit pAb (A2845)

Product:

Bcl-2 Rabbit pAb

Journal:

BMC medicine

Application:

IF:

Species:

Mus musculus

PMID:

30782145

Title:

Cathelicidin-related antimicrobial peptide protects against myocardial ischemia/reperfusion injury.

References for Bcl-2 Rabbit pAb (A2845)

Product:

Bcl-2 Rabbit pAb

Journal:

BMC Res Notes

Application:

IF:

Species:

Homo sapiens

PMID:

32807213

Title:

Levobupivacaine inhibits proliferation and promotes apoptosis of breast cancer cells through PI3K/Akt/mTOR signalling pathway.

References for Bcl-2 Rabbit pAb (A2845)

Product:

Bcl-2 Rabbit pAb

Journal:

Mol Cell Biochem

Application:

IF:

3.5

Species:

Homo sapiens

PMID:

32394311

Title:

Astragaloside IV protects human cardiomyocytes from hypoxia/reoxygenation injury by regulating miR-101a

References for Bcl-2 Rabbit pAb (A2845)

Product:

Bcl-2 Rabbit pAb

Journal:

Journal of cardiovascular translational research

Application:

IF:

2.4

Species:

Mus musculus

PMID:

37036598

Title:

Danlou Tablet Protects Against Cardiac Remodeling and Dysfunction after Myocardial Ischemia/Reperfusion Injury through Activating AKT/FoxO3a Pathway

References for Bcl-2 Rabbit pAb (A2845)

Product:

Bcl-2 Rabbit pAb

Journal:

Journal of cardiovascular translational research

Application:

IF:

2.4

Species:

Mus musculus

PMID:

37784003

Title:

Danlou Tablet Protects Against Myocardial Infarction Through Promoting eNOS-Dependent Endothelial Protection and Angiogenesis

References for Bcl-2 Rabbit pAb (A2845)

Product:

Bcl-2 Rabbit pAb

Journal:

Oncology Letters

Application:

IF:

2.5

Species:

Homo sapiens

PMID:

31612057

Title:

Propyl isothiocyanate induces apoptosis in gastric cancer cells by oxidative stress via glutathione depletion

References for Bcl-2 Rabbit pAb (A2845)

Product:

Bcl-2 Rabbit pAb

Journal:

Oncology letters

Application:

IF:

2.5

Species:

Homo sapiens

PMID:

31612057

Title:

Propyl isothiocyanate induces apoptosis in gastric cancer cells by oxidative stress via glutathione depletion

References for Bcl-2 Rabbit pAb (A2845)

Product:

Bcl-2 Rabbit pAb

Journal:

Research (Washington, D.C.)

Application:

IF:

Species:

Rattus norvegicus

PMID:

38410280

Title:

Exercise-Induced miR-210 Promotes Cardiomyocyte Proliferation and Survival and Mediates Exercise-Induced Cardiac Protection against Ischemia/Reperfusion Injury - PMC

References for Bcl-2 Rabbit pAb (A2845)

Product:

Bcl-2 Rabbit pAb

Journal:

Cell Death Differ

Application:

IF:

13.7

Species:

Mus musculus

PMID:

34413485