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Phospho-AMPKa1-T183 + AMPKa2-T172 Rabbit mAb (AP1345)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Western blot analysis of lysates from C2C12 cells, using Phospho-AMPKa1-T183 + AMPKa2-T172 Rabbit mAb (AP1345) at1:1000 dilution. C2C12 cells were treated by oligomycin (0. 5 uM) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product name	Phospho-AMPKa1-T183 + AMPKa2-T172 Rabbit mAb
Catalog No.	AP1345
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC53386

Background

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around T183 of human AMPKa1 (NP_006242.5).
Sequence	LRTSC
Gene ID
Swiss Prot
Synonyms	AMPKa1/AMPKa2; Phospho-AMPKa1-T183 + AMPKa2-T172
Calculated MW	64kDa/65kDa/62kDa
Observed MW	62kDa

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	C2C12 treated by oligomycin
Cellular location	Apical plasma membrane, Cytoplasm, Cytosol, Nuclear speck, Nucleoplasm, nucleus.
Customer validation	WB(Sus scrofa, Gallus gallus)