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Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0883)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Rat

Western blot analysis of various lysates using Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0883) at 1:1000 dilution. 293T and C6 cells were treated by ATP(5 mM) at 30℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Overview

Product name	Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb
Catalog No.	AP0883
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around T183 of human PRKAA1PRKAA1 (NP_006242.5).
Sequence	LRTSC
Gene ID
Swiss Prot
Synonyms	AMPKa1/AMPKa2; Phospho-AMPKa1-T183/AMPKa2-T172
Calculated MW	64kDa/65kDa/62kDa
Observed MW	62kDa

Applications

Reactivity	Human, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	293T, C6
Cellular location	apical plasma membrane, cytoplasm, cytosol, nuclear speck, nucleoplasm, nucleus.
Customer validation	WB(Rattus norvegicus, Mus musculus, Homo sapiens)