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Phospho-HSL-S660 Rabbit mAb (AP1432)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Western blot analysis of lysates from 3T3-L1 cells, using Phospho-HSL-S660 Rabbit mAb (AP1432) at 1:1000 dilution. 3T3-L1 cells that were not differentiated or differentiated into adipocytes treated with 0. 06mol/L alcohol and treated with isoproterenol (10 μM, 20 min).
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product name	Phospho-HSL-S660 Rabbit mAb
Catalog No.	AP1432
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC61254

Background

The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.

Immunogen information

Immunogen	A synthesized peptide derived from human HSL (Phospho-Ser660)
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	HSL; LHS; REH; AOMS4; FPLD6; Phospho-HSL-S660
Calculated MW	117kDa
Observed MW	84kDa

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	3T3-L1 treated with isoproterenol
Cellular location	Cell membrane, Cytoplasm, Membrane, caveola, cytosol.
Customer validation	WB(Mus musculus)