제품 > 항체 > 인산화 특이적 항체

Phospho-PPP2CA/PPP2CB-Y307 Rabbit pAb (AP0927)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates using Phospho-PPP2CA/PPP2CB-Y307 Rabbit pAb (AP0927) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name	Phospho-PPP2CA/PPP2CB-Y307 Rabbit pAb
Catalog No.	AP0927
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]

Immunogen information

Immunogen	A synthetic phosphorylated peptide around Y307 of human PPP2CA/PPP2CBPPP2CB (NP_001009552.1).
Sequence	PDYFL
Gene ID
Swiss Prot
Synonyms	PPP2CA/PPP2CB; Phospho-PPP2CA/PPP2CB-Y307
Calculated MW
Observed MW	38kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa treated by Calyculin A, NIH/3T3 treated by Calyculin A
Cellular location	cytosol, extracellular exosome, microtubule cytoskeleton, mitochondrion, nucleus, plasma membrane, spindle pole, synapse.
Customer validation	WB(Mus musculus, Homo sapiens) IF(Homo sapiens) ChIP(Homo sapiens) DB(Homo sapiens)