제품 > 항체 > 단일클론항체(mAb)

active Caspase-3 Rabbit mAb (A11021)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from Jurkat cells, using Active Caspase-3 Rabbit mAb (A11021) at 1:500 dilution. Jurkat cells were treated by Staurosporine(1 μM) at room temperature for 3 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Review

Overview

Product name	active Caspase-3 Rabbit mAb
Catalog No.	A11021
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0185

Background

The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CASP3 (P42574).
Sequence	MENTENSVDSKSIKNLEPKIIHGSESMDSGISLDNSYKMDYPEMGLCIIINNKNFHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELM
Gene ID
Swiss Prot
Synonyms	CPP32; SCA-1; CPP32B; Active Caspase-3
Calculated MW	32kDa
Observed MW	17kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Jurkat treated by Staurosporine
Cellular location	Cytoplasm.

Documents

Certificate of Compliance

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Lot number

Research Area

active Caspase-3 Rabbit mAb images

Western blot - active Caspase-3 Rabbit mAb (A11021)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A11021

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Reviews List

Product Protocols

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

FASEB J

Application:

IF:

5.191

Species:

Homo sapiens

PMID:

32539222

Title:

Src‐mediated phosphorylation of GAPDH regulates its nuclear localization and cellular response to DNA damage

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

2020 Jul Chin J Nat Med

Application:

IF:

2.343

Species:

Homo sapiens

PMID:

32616189

Title:

GL-V9 reverses adriamycin resistance in hepatocellular carcinoma cells by affecting JNK2-related autophagy

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Toxics

Application:

IF:

4.146

Species:

Mus musculus

PMID:

32751088

Title:

Increased Mitochondrial Fragmentation Mediated by Dynamin-Related Protein 1 Contributes to Hexavalent Chromium-Induced Mitochondrial Respiratory Chain Complex I-Dependent Cytotoxicity

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Oncol Rep

Application:

IF:

3.906

Species:

Homo sapiens

PMID:

32945513

Title:

Curcumin enhances anti-ancer efficacy of either gemcitabine or docetaxel on pancreatic cancer cells

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Pathogens

Application:

IF:

3.492

Species:

Giardia duodenalis

PMID:

32842537

Title:

Giardia duodenalis Induces Apoptosis in Intestinal Epithelial Cells via Reactive Oxygen Species-Mediated Mitochondrial Pathway In Vitro

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Food Chem Toxicol

Application:

IF:

6.023

Species:

Homo sapiens

PMID:

32619556

Title:

Glycolysis inhibition and apoptosis induction in human prostate cancer cells by FV-429-mediated regulation of AR-AKT-HK2 signaling network

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Placenta

Application:

IF:

3.481

Species:

Mus musculus

PMID:

33068962

Title:

The role of β-HCG and VEGF-MEK/ERK signaling pathway in villi angiogenesis in patients with missed abortion

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Cell Biol Toxicol

Application:

IF:

6.691

Species:

Homo sapiens

PMID:

33126266

Title:

Induction of cell cycle arrest and apoptosis by CPUC002 through stabilization of p53 and suppression of STAT3 signaling pathway in multiple myeloma

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Cancer Lett

Application:

IF:

8.679

Species:

Homo sapiens

PMID:

33129955

Title:

A novel kinase inhibitor, LZT-106, downregulates Mcl-1 and sensitizes colorectal cancer cells to BH3 mimetic ABT-199 by targeting CDK9 and GSK-3β signaling

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Genes

Application:

IF:

4.096

Species:

Homo sapiens

PMID:

33158173

Title:

Lymphatic Metastasis of NSCLC Involves Chemotaxis Effects of Lymphatic Endothelial Cells through the CCR7–CCL21 Axis Modulated by TNF-α

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Cancer Cell

Application:

IHC

IF:

38.585

Species:

Mus musculus

PMID:

32049046

Title:

Direct Phosphorylation and Stabilization of MYC by Aurora B Kinase Promote T-cell Leukemogenesis

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Phytomedicine : international journal of phytotherapy and phytopharmacology

Application:

IF:

6.65

Species:

Homo sapiens

PMID:

36115171

Title:

Effects of natural 24-epibrassinolide on inducing apoptosis and restricting metabolism in hepatocarcinoma cells

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Molecular medicine reports

Application:

IF:

3.42

Species:

Homo sapiens

PMID:

31974619

Title:

Chidamide induces necroptosis via regulation of c‑FLIPL expression in Jurkat and HUT‑78 cells

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Toxicology

Application:

IF:

4.57

Species:

Homo sapiens

PMID:

36243239

Title:

Maternal exposure to PM2. 5 decreases ovarian reserve in neonatal offspring mice through activating PI3K/AKT/FoxO3a pathway and ROS-dependent NF-κB pathway

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Acta pharmaceutica Sinica. B

Application:

IF:

14.9

Species:

Mus musculus

PMID:

35127378

Title:

Astaxanthine attenuates cisplatin ototoxicity in vitro and protects against cisplatin-induced hearing loss in vivo

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Frontiers in bioscience (Landmark edition)

Application:

IF:

3.11

Species:

Sus scrofa,Mesocricetus auratus

PMID:

36224019

Title:

LncRNA-SUSAJ1 Activates the ER Stress Pathway Inhibiting JEV Proliferation by Promoting PK15 Cells Apoptosis

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Anticancer research

Application:

IF:

2.43

Species:

Homo sapiens

PMID:

36854527

Title:

Hinokitiol Inhibits the Viability of Oral Squamous Carcinoma Cells by Inducing Apoptosis and Autophagy

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Molecules (Basel, Switzerland)

Application:

IF:

4.92

Species:

Homo sapiens

PMID:

36838737

Title:

Luteoloside Induces G0/G1 Phase Arrest of Neuroblastoma Cells by Targeting p38 MAPK - PMC

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Scientific reports

Application:

IF:

4.99

Species:

Homo sapiens

PMID:

37217794

Title:

LncRNA FAS-AS1 upregulated by its genetic variation rs6586163 promotes cell apoptosis in nasopharyngeal carcinoma through regulating mitochondria function and Fas splicing - PMC

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Molecular medicine (Cambridge, Mass.)

Application:

IF:

6.37

Species:

Homo sapiens

PMID:

37626284

Title:

Elevated galectin-3 levels detected in women with hyperglycemia during early and mid-pregnancy antagonizes high glucose-induced trophoblast cells apoptosis via galectin-3/foxc1 pathway - PMC

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Cardiovascular diabetology

Application:

IF:

8.94

Species:

Mus musculus

PMID:

37891556

Title:

Klotho inhibits renal ox-LDL deposition via IGF-1R/RAC1/OLR1 signaling to ameliorate podocyte injury in diabetic kidney disease - PMC

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Chemical & pharmaceutical bulletin

Application:

IF:

1.9

Species:

Homo sapiens

PMID:

37914257

Title:

Semisynthesis and in Vitro Anti-cancer Effect of New Magnolol Derivatives on the Cell Proliferation, Apoptosis, Migration, and Invasion of Human Hepatocellular

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

IF:

7.41

Species:

Homo sapiens

PMID:

37633053

Title:

Inhibition of mitochondrial fusion via SIRT1/PDK2/PARL axis breaks mitochondrial metabolic plasticity and sensitizes cancer cells to glucose restriction

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Cancer cell international

Application:

IF:

6.429

Species:

Homo sapiens

PMID:

35264157

Title:

Novel sequential therapy with metformin enhances the effects of cisplatin in testicular germ cell tumours via YAP1 signalling

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

ACS nano

Application:

IF:

18.02

Species:

Homo sapiens

PMID:

37099675

Title:

Tailored Apoptotic Vesicle Delivery Platform for Inflammatory Regulation and Tissue Repair to Ameliorate Ischemic Stroke

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Medicina (Kaunas, Lithuania)

Application:

IHC

IF:

2.94

Species:

Rattus norvegicus

PMID:

38138222

Title:

Amentoflavone Mitigates Cyclophosphamide-Induced Pulmonary Toxicity: Involvement of -SIRT-1/Nrf2/Keap1 Axis, JAK-2/STAT-3 Signaling, and Apoptosis - PMC

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Application:

IF:

5.57

Species:

Homo sapiens, Mus musculus

PMID:

36758785

Title:

Ganoderma lucidum extract-mediated gasdermin E cleavage promotes tumor cell pyroptosis and inhibits metastasis in breast cancer

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

European journal of pharmacology

Application:

IF:

5.19

Species:

Homo sapiens

PMID:

38061471

Title:

Tetrahydroalstonine possesses protective potentials on palmitic acid stimulated SK-N-MC cells by suppression of A1-42 and tau through regulation of PI3K/Akt

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Autophagy

Application:

IF:

13.39

Species:

Homo sapiens

PMID:

37471054

Title:

Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

iScience

Application:

IF, WB

IF:

5.8

Species:

Homo sapiens

PMID:

38439974

Title:

GL-V9 inhibits the activation of AR-AKT-HK2 signaling networks and induces prostate cancer cell apoptosis through mitochondria-mediated mechanism - PMC

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

ACS nano

Application:

IF:

17.1

Species:

Mus musculus

PMID:

38266247

Title:

Bioactive Ceria Nanoenzymes Target Mitochondria in Reperfusion Injury to Treat Ischemic Stroke

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Free radical biology & medicine

Application:

IF:

7.4

Species:

Homo sapiens,Mus musculus

PMID:

38461872

Title:

The long coiled-coil protein NECC2 regulates oxLDL-induced endothelial oxidative damage and exacerbates atherosclerosis development in apolipoprotein E

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Cell death & disease

Application:

IF:

Species:

Mus musculus

PMID:

38378757

Title:

HRD1-induced TMEM2 ubiquitination promotes ER stress-mediated apoptosis through a non-canonical pathway in intestinal ischemia/reperfusion - PMC

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Acta pharmaceutica Sinica. B

Application:

IF:

14.5

Species:

Mus musculus

PMID:

38572095

Title:

Eubacterium coprostanoligenes alleviates chemotherapy-induced intestinal mucositis by enhancing intestinal mucus barrier

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Biochimica et biophysica acta. Molecular basis of disease

Application:

IF:

6.633

Species:

Rattus norvegicus

PMID:

30579931

Title:

SIRT1 activation by SRT1720 attenuates bone cancer pain via preventing Drp1-mediated mitochondrial fission.

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Acta Pharm Sin B

Application:

IF:

14.903

Species:

Homo sapiens

PMID:

34729308

Title:

Cholesterol-associated lysosomal disorder triggers cell death of hematological malignancy: Dynamic analysis on cytotoxic effects of LW-218

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Science advances

Application:

Other

IF:

14.95

Species:

Homo sapiens

PMID:

36638165

Title:

The role of altered lipid composition and distribution in liver fibrosis revealed by multimodal nonlinear optical microscopy - PMC

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

The Journal of biological chemistry

Application:

IF:

Species:

Homo sapiens

PMID:

38705394

Title:

Hhatl ameliorates endoplasmic reticulum stress through autophagy by associating with LC3

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

The Journal of pharmacy and pharmacology

Application:

IF:

3.3

Species:

Homo sapiens

PMID:

38767973

Title:

GL-V9 synergizes with oxaliplatin of colorectal cancer via Wee1 degradation mediated by HSP90 inhibition

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Theranostics

Application:

IF:

12.4

Species:

Homo sapiens, Mus musculus

PMID:

33391463

Title:

LT-171-861, a novel FLT3 inhibitor, shows excellent preclinical efficacy for the treatment of FLT3 mutant acute myeloid leukemia

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Frontiers in Pharmacology

Application:

IF:

5.6

Species:

Homo sapiens,Rattus norvegicus

PMID:

38562460

Title:

Isoquercitrin alleviates pirarubicin-induced cardiotoxicity in vivo and in vitro by inhibiting apoptosis through Phlpp1/AKT/Bcl-2 signaling pathway - PMC

References for active Caspase-3 Rabbit mAb (A11021)

Product:

active Caspase-3 Rabbit mAb

Journal:

Journal for immunotherapy of cancer

Application:

IF:

10.9

Species:

Homo sapiens

PMID:

39357981