제품 > 항체 > 다중클론항체(pAb)

Bcl-2 Rabbit pAb (A16776)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Bcl-2 Rabbit pAb (A16776) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Western blot analysis of lysates from HCT116 cells, using Bcl-2 Rabbit pAb (A16776) at 1:1000 dilution. HCT116 cells treated with different concentrations of IL-8.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Bcl-2 Rabbit pAb (A16776)

Immunohistochemistry analysis of paraffin-embedded Human stomach using Bcl-2 Rabbit pAb (A16776) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product name	Bcl-2 Rabbit pAb
Catalog No.	A16776
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bcl-2 (NP_000624.2).
Sequence	MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAPAPGIFSSQPGHTPHPAASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQA
Gene ID
Swiss Prot
Synonyms	Bcl-2; PPP1R50
Calculated MW	26kDa
Observed MW	24kDa/26kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	293T, HeLa, mouse lung, mouse testis, HCT116 treated with IL-8
Cellular location	Endoplasmic reticulum membrane, Mitochondrion outer membrane, Nucleus membrane, Single-pass membrane protein.
Customer validation	IF(Rattus norvegicus) WB(Mus musculus, Homo sapiens, Capra hircus) IHC(Mus musculus, Rattus norvegicus, Homo sapiens)

Documents

Certificate of Compliance

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Research Area

Bcl-2 Rabbit pAb images

Western blot - Bcl-2 Rabbit pAb (A16776)

Immunohistochemistry - Bcl-2 Rabbit pAb (A16776)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Bcl-2 Rabbit pAb (A16776)

Product:

Bcl-2 Rabbit pAb

Journal:

Cell and tissue research

Application:

IF:

3.2

Species:

Rattus norvegicus

PMID:

31377879

Title:

Effect of silencing HIF-1α gene on testicle spermatogenesis function in varicocele rats

References for Bcl-2 Rabbit pAb (A16776)

Product:

Bcl-2 Rabbit pAb

Journal:

Exp Ther Med

Application:

IF:

2.4

Species:

Mus musculus

PMID:

33680107

Title:

Schisantherin A improves learning and memory abilities partly through regulating the Nrf2/Keap1/ARE signaling pathway in chronic fatigue mice

References for Bcl-2 Rabbit pAb (A16776)

Product:

Bcl-2 Rabbit pAb

Journal:

Asian Pacific Journal of Cancer Prevention

Application:

IF:

Species:

Homo sapiens

PMID:

31244309

Title:

Role of TMPRSS4 Modulation in Breast Cancer Cell Proliferation.

References for Bcl-2 Rabbit pAb (A16776)

Product:

Bcl-2 Rabbit pAb

Journal:

Asian Pacific Journal of Cancer Prevention

Application:

IF:

Species:

Homo sapiens

PMID:

31870109

Title:

Downregulation of TMPRSS4 Enhances Triple-Negative Breast Cancer Cell Radiosensitivity Through Cell Cycle and Cell Apoptosis Process Impairment.

References for Bcl-2 Rabbit pAb (A16776)

Product:

Bcl-2 Rabbit pAb

Journal:

Anim Biotechnol

Application:

IF:

1.7

Species:

Capra hircus

PMID:

32730101

Title:

Pou2F3 silencing enhanced the proliferation of mammary epithelial cells in dairy goat via PI3K/AKT/mTOR signaling pathway

References for Bcl-2 Rabbit pAb (A16776)

Product:

Bcl-2 Rabbit pAb

Journal:

Biomedicines

Application:

IHC

IF:

4.7

Species:

Mus musculus

PMID:

37189672

Title:

Streptomyces Bioactive Metabolites Prevent Liver Cancer through Apoptosis, Inhibiting Oxidative Stress and Inflammatory Markers in Diethylnitrosamine-Induced Hepatocellular Carcinoma - PMC

References for Bcl-2 Rabbit pAb (A16776)

Product:

Bcl-2 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

5.6

Species:

Capra hircus

PMID:

37240097

Title:

miR-486 Responds to Apoptosis and Autophagy by Repressing SRSF3 Expression in Ovarian Granulosa Cells of Dairy Goats

References for Bcl-2 Rabbit pAb (A16776)

Product:

Bcl-2 Rabbit pAb

Journal:

Toxicology and applied pharmacology

Application:

IHC

IF:

3.3

Species:

Rattus norvegicus

PMID:

38040297

Title:

Saroglitazar mitigated NASH-associated hepatic injury in dexamethasone-treated rats via modulating autophagy, apoptosis, and necroptosis

References for Bcl-2 Rabbit pAb (A16776)

Product:

Bcl-2 Rabbit pAb

Journal:

Pharmaceuticals (Basel, Switzerland)

Application:

IHC

IF:

4.3

Species:

Homo sapiens

PMID:

39598348

Title:

Molecular Docking, Bioinformatic Analysis, and Experimental Verification for the Effect of Naringin on ADHD: Possible Inhibition of GSK-3 and HSP90 - PMC